This project is part of research projects related with denitrification process made by the Department of Sanitation and Environment of the Architecture and Civil Engineering Faculty of Unicamp. Anaerobic filter filled with synthetic material and UASB reactor, both in lab-scale, will be fed with synthetic effluent enriched with nitrate at concentrations 0, 25, 50 and 75 mg N-NO3- L-1 to compare nitrogen removal efficiency between both reactors. Moreover, for a better understanding of the systems studied, the hydrodynamic characteristics of the reactors will be evaluated for each nitrate concentration studied, as well as the biogas produced, which will be analyzed by gas chromatography in terms of methane, carbon dioxide and nitrous oxide, considered greenhouse gases. Finally, to understand the present microbiology in the reactors, sludge and biofilm will be evaluated using Fluorescent in situ hybridization (FISH) in order to quantify the group of Proteobacteria and Archeas, groups including the denitrifying and methanogenic respectively. All analyzes of the collected samples will be based on Standard Methods for the Examination of Water and Wastewater.
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