| Grant number: | 14/21910-8 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | October 01, 2015 |
| End date: | August 31, 2018 |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry - Physical-Chemistry |
| Principal Investigator: | Mateus Borba Cardoso |
| Grantee: | Agustín Silvio Picco |
| Host Institution: | Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil |
Abstract The purpose of this project is to study the evolution of silica nanoparticles in biological media by addressing the protein corona formation, aggregation process and the connection between these two events with the cellular uptake. Silica nanoparticles of different diameters with distinct surface chemistries will be synthesized in order to establish a cross-correlation between size and surface properties with the nanoparticles behavior in biological fluids. The nanoparticles will be fully characterized by Dynamic Light Scattering (DLS), Zeta Potential, Small Angle X-Ray Scattering (SAXS), Transmission Electron Microscopy (TEM), Thermogravimetric Analysis (TGA), Fourier Transform Infrared Spectroscopy (FT-IR) and Nitrogen adsorption/desorption isotherms. In addition to these techniques, electrophoresis (SDS-PAGE) and liquid chromatography mass spectroscopy (LC-MS) will be performed to obtain information on the constitution of protein corona while time-resolved SAXS will be used to understand the aggregation behavior. Finally, the impact of protein corona and aggregation on cellular uptake and cytotoxicity will be followed by means of Confocal Microscopy and Perkin Elmer Operetta/Flow Cytometry techniques. | |
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