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Anti-IL-17 in the modulation of lung mechanics and inflammation in mice with acute lung injury induced by LPS

Grant number: 15/07150-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2015
Effective date (End): September 30, 2016
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Iolanda de Fátima Lopes Calvo Tibério
Grantee:Marcus Vinicius Rodrigues de Agrela
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Th17 cytokines seem to play an important role in the pathogenesis of acute lung injury (ALI), although its exact influence is uncertain. Studies have demonstrated an influence of these cytokines in modulating inflammation or infection. Many of the effects of this pathway have not been investigated in models with acute lung injury (ALI). In the present study will evaluate the effects of treatment with anti-IL-17 in a model of ALI induced by lipopolysaccharide (LPS). We assess lung mechanics, inflammatory response in the airways and in the distal lung of mice chronically exposed to ovalbumin or lung injury induced by LPS. The BALB/c mice submitted to LPA will be anesthetized with isoflurane and will receive single intratracheal instillation of LPS diluted in saline at a dose of 5mg/kg. The treatment groups will receive a single dose of anti-IL-17 hour before exposure to LPS and control groups receive saline. Twenty-four hours after the end of the experimental protocol, the animals will be subject to an assessment of lung mechanics. Then it will be made histological analyzes for numbers of eosinophils and cytokines (IL-6, IL-10, IL-13, IL -17) in the different groups.