Screening of drugs similar to chalcones and curcuminas in the model of the Caenorhabditis elegans

• Grant number: 15/18745-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): November 01, 2015
• Effective date (End): December 31, 2016
• Field of knowledge: Biological Sciences - Parasitology - Helminthology of Parasites
• Principal Investigator: Fernanda de Freitas Anibal
• Grantee: Yulli Roxenne Albuquerque
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Resistance to various anthelmintic drugs is already observed in many infected animals and can get to become a serious problem for human health in the near future. Compounds derivatives of chalcones and curcumins have been used as an excellent source to search for innovative therapeutic agents. The objective of this study is to evaluate of the anthelmintic activity of synthetic compounds counterparts to chalcones and curcumins in model organism Caenorhabditis elegans. The nematode C. elegans will be cultivated and maintained amid NGM (Nematode Growth Medium) at 20 ° C. Adult nematodes will be transferred to 24 well plates containing NGM where they will be exposed, after 24 hours, 40 uL of different concentrations (25 uM, 50 uM, 100 uM, 200 uM, 300 uM, 400 uM and 500 uM) of compounds synthetic A1K2, A2K2, A8K2 and A11K2 and curcumin. The nematicide activity of each drug will be observed in stereomicroscope in periods of 6h, 24h, 30h and 48h after application of drugs through the worm count that remained alive, noting the change in motility and the appearance of eggs and larvae of worms in wells. All tests will be performed in triplicate. It will be used 10% DMSO (dimethylsulfoxide) as the negative control and the Albendazole (40 mg/ml) as the positive control. The datas will be analyzed using the SPSS-19® by ANOVA (Compare means) and the significance level will be set at 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALBUQUERQUE, YULLI R.; PRATAVIEIRA, SEBASTIAO; BAGNATO, VANDERLEI S.; INADA, NATALIA M.; SOUSA, LARISSA M.; AFONSO, ANA; SOUZA, CLOVIS W. O.; OLIVEIRA, KLEBER T.; ANIBAL, FERNANDA F.; KESSEL, DH; et al. Photodynamic inactivation using curcuminoids and Photogem (R) on Caenorhabditis elegans. OPTICAL METHODS FOR TUMOR TREATMENT AND DETECTION: MECHANISMS AND TECHNIQUES IN PHOTODYNAMIC THERAPY XXVII, v. 10476, p. 7-pg., 2018-01-01. (15/18745-8, 09/54035-4, 13/07276-1, 14/50857-8)