PROTEOMIC ANALYSIS OF ACQUIRED PELLICLE FORMED ON EXPERIMENTAL COMPOSITE RESINS CONTAINING OR NOT FILLER AND/OR PROTEASE INHIBITORS

• Grant number: 14/25599-5
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): December 01, 2015
• Effective date (End): March 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Vinícius Taioqui Pelá
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Polymeric materials, especially composite resins, have been extensively employed and new formulations have been often proposed. Acquired pellicle is a film constituted mainly of salivary proteins of glycoproteins that deposit onto the surfaces that are exposed to the oral environment. However, the composition of the acquired pellicle formed on surfaces of composite resins was never studies. Also changes in the composition of the acquired pellicle when the composition of the resin materials is changed was not studied before. Thus, this study will evaluate the influence of the addition of inorganic filler and/or protease inhibitors to experimental composite resins on the protein composition of the acquired pellicle formed on the resin specimens. Specimens (5 mm diameter and 3 mm thickness) will be prepared with Bis-GMA and TEGDMA (70/30 mol%)experimental composite resins. These specimens will be divided into 2 maisn groups (n=60 per group), one with the addition of inorganic filler and the other with 70% inorganic filler (in weight). Each group will be divided into 3 subgroups (n=20 per subgroup) that will differ with respect to the absence or presence of protease inhibitor(1% EGCG or 1% chlorhexidine. Stimulated saliva will be collected from 3 volunteers and used to form acquired pellicle on the specimens of composite resin (100 µL per specimens, for 2 h). The pellicle will be collected with wick filters pre-soaked in 3% citric acid and processed by analysis through nLC-ESI-MS/MS.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PELA, VINICIUS TAIOQUI; SILVA CASSIANO, LUIZA PAULA; DA SILVA VENTURA, TALITA MENDES; DE SOUZA-E-SILVA, CINTIA MARIA; GIRONDA, CARLOS CONDARCO; RIOS, DANIELA; RABELO BUZALAF, MARILIA AFONSO. Proteomic analysis of the acquired enamel pellicle formed on human and bovine tooth: a study using the Bauru in situ pellicle model (BISPM). Journal of Applied Oral Science, v. 27, 2019. (14/25599-5)

PELA, VINICIUS TAIOQUI; PRAKKI, ANURADHA; WANG, LINDA; SILVA VENTURA, TALITA MENDES; DE SOUZA E SILVA, CINTIA MARIA; SILVA CASSIANO, LUIZA PAULA; FREITAS BRIANEZZI, LETICIA FERREIRA; LEITE, ALINE LIMA; RABELO BUZALAF, MARILIA AFONSO. The influence of fillers and protease inhibitors in experimental resins in the protein profile of the acquired pellicle formed in situ on enamel-resin specimens. ARCHIVES OF ORAL BIOLOGY, v. 108, DEC 2019. (14/25599-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PELÁ, Vinícius Taioqui. Proteomic analysis of acquired pellicle formed on experimental composite resins containing or not filler and/or protease inhibitors: study in situ. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.