Fingerprint de metabólitos secundários em extratos de cumaru (Dipteryx odorata) e Guaçatonga (Casearia sylvestris)

• Grant number: 15/24086-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2016
• End date: January 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Maria de Lourdes Leite de Moraes
• Grantee: Thais Codogno Franco
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

The metabolic profile of a plant extract gives us information about the biologically active compounds present in the extract. This fingerprint has encouraged integrated approaches able to provide information about the synergy and interactions involved in the pharmacological responses of bioactive complex matrices. Capillary electrophoresis (CE) protocols has been used in our group for analysis of crude herbal extracts providing satisfactory results. This project aims to establish the electrophoretic profile of secondary metabolites present in polar and nonpolar extracts of tonka bean (Dipteryx odorata) and in Guacatonga (Casearia sylvestris) leaves, using CE protocols to assess alkaloids, carboxylic acids and fatty acids. Phenolic compounds of these plants have been studied in previous works. Tonka bean shows high commercial value due to the high content of coumarin present (3-10%), and the extract has been used in the pharmaceutical, cosmetic and food industries. Despite this, to date, there are few reports about the total composition of secondary metabolites of the seed extract. Guacatonga is a great accent plant in folk medicine due to its anti-inflammatory, antitumor and antiulcerogenic properties. The main chemical compound found are the casearins. Several studies have been conducted to determine the casearins of the Guacatonga, however, it has not been done the fingerprint of its extract, assessing other metabolites.