The role of Rock protein on glucose uptake in the skeletal muscle of exercised rodents during the aging process

• Grant number: 15/26000-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2016
• End date: August 29, 2018
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: José Rodrigo Pauli
• Grantee: Vitor Rosetto Muñoz
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated scholarship(s): 17/17106-7 - The role of brown adipose tissue (BAT) on cardiovascular function in aged mice, BE.EP.MS

Abstract

Senescence is associated to several metabolic disorders, including insulin resistance (IR). Physical exercise has an important role as non­pharmacological agent in the prevention and treatment of IR and the associated complications. Rho­kinase (Rock) has been postulated as an important protein that acts directly on the phosphorylation of IRS­1, contributing up to 50% of glucose uptake in skeletal muscle. Therefore, the objective of this study is to evaluate the role of physical exercise on Rock expression and glucose uptake in aged rats. Fischer 344 rats will be distributed into three groups: Sedentary Young (5 months) Middle Age (17 months) and Old (21 months). The MA and Old groups will be submitted to chronic (5 days) or acute swimming exercise (1 session) protocols, composed by 4-30 minutes swimming sessions with 5 minutes of rest between them. After the first session, a 1.5% of body weight workload will be attached to animal´s thorax for the last three bouts. Part of animals will submitted to the insulin tolerance test, another to the glucose tolerance test. Sixteen hours after the exercise session, the last animals will be euthanized for insulin signaling pathway and Rock metabolism analysis through immunoprecipitation, western blotting and Rock-activity assay techniques.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUNOZ, VITOR ROSETTO; GASPAR, RAFAEL CALAIS; MINUZZI, LUCIELE GUERRA; CANCIGLIERI, RAPHAEL DOS SANTOS; RAMOS DA SILVA, ADELINO SANCHEZ; DE MOURA, LEANDRO PEREIRA; CINTRA, DENNYS ESPER; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO. Rho-kinase activity is upregulated in the skeletal muscle of aged exercised rats. Experimental Gerontology, v. 128, DEC 2019. (18/20872-6, 15/26000-2, 16/18488-8)

MUNOZ, VITOR R.; GASPAR, RAFAEL C.; SEVERINO, MATHEUS B.; MACEDO, ANA P. A.; SIMABUCO, FERNANDO M.; ROPELLE, EDUARDO R.; CINTRA, DENNYS E.; DA SILVA, ADELINO S. R.; KIM, YOUNG-BUM; PAULI, JOSE RODRIGO. Exercise Counterbalances Rho/ROCK2 Signaling Impairment in the Skeletal Muscle and Ameliorates Insulin Sensitivity in Obese Mice. FRONTIERS IN IMMUNOLOGY, v. 12, JUN 21 2021. (16/18488-8, 13/21491-2, 15/26000-2)

MUNOZ, VITOR R.; GASPAR, RAFAEL C.; KUGA, GABRIEL K.; NAKANDAKARI, SUSANA C. B. R.; BAPTISTA, IGOR L.; MEKARY, RANIA A.; DA SILVA, ADELINO S. R.; DE MOURA, LEANDRO P.; ROPELLE, EDUARDO R.; CINTRA, DENNYS E.; et al. Exercise decreases CLK2 in the liver of obese mice and prevents hepatic fat accumulation. Journal of Cellular Biochemistry, v. 119, n. 7, p. 5885-5892, JUL 2018. (15/26000-2)

MUNOZ, VITOR ROSETTO; BOTEZELLI, JOSE DIEGO; GASPAR, RAFAEL CALAIS; DA ROCHA, ALISSON L.; VIEIRA, RENAN FUDOLI LINS; CRISOL, BARBARA MOREIRA; BRAGA, RENATA ROSSETO; SEVERINO, MATHEUS BRANDEMARTE; NAKANDAKARI, SUSANA CASTELO BRANCO RAMOS; ANTUNES, GABRIEL CALHEIROS; et al. Effects of short-term endurance and strength exercise in the molecular regulation of skeletal muscle in hyperinsulinemic and hyperglycemic Slc2a4(+/-) mice. CELLULAR AND MOLECULAR LIFE SCIENCES, v. 80, n. 5, p. 16-pg., 2023-05-01. (16/18488-8, 21/08692-5, 15/26000-2, 19/11820-5, 20/13443-1, 18/20872-6)

MUNOZ, VITOR ROSETTO; GASPAR, RAFAEL CALAIS; ESTECA, MARCOS VINICIUS; BAPTISTA, IGOR LUCHINI; LINS VIEIRA, RENAN FUDOLI; RAMOS DA SILVA, ADELINO SANCHEZ; DE MOURA, LEANDRO PEREIRA; CINTRA, DENNYS ESPER; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO. Physical exercise increases ROCK activity in the skeletal muscle of middle-aged rats. MECHANISMS OF AGEING AND DEVELOPMENT, v. 186, MAR 2020. (16/18488-8, 15/26000-2, 18/20872-6)

MUNOZ, VITOR ROSETTO; GASPAR, RAFAEL CALAIS; KUGA, GABRIEL KEINE; BETIM PAVAN, ISADORA CAROLINA; SIMABUCO, FERNANDO MOREIRA; RAMOS DA SILVA, ADELINO SANCHEZ; DE MOURA, LEANDRO PEREIRA; CINTRA, DENNYS ESPER; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO. The Effects of Aging on Rho-Kinase and Insulin Signaling in Skeletal Muscle and White Adipose Tissue of Rats. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, v. 75, n. 3, p. 432-436, MAR 2020. (12/13558-7, 16/18488-8, 15/26000-2)

MUNOZ, V. R.; GASPAR, R. C.; MANCINI, M. C. S.; DE LIMA, R. D.; VIEIRA, R. F. L.; CRISOL, B. M.; ANTUNES, G. C.; TROMBETA, J. C. S.; BONFANTE, I. L. P.; SIMABUCO, F. M.; et al. Short-term physical exercise controls age-related hyperinsulinemia and improves hepatic metabolism in aged rodents. Journal of Endocrinological Investigation, v. N/A, p. 13-pg., 2022-11-01. (16/18488-8, 19/11820-5, 18/20872-6, 21/08692-5, 20/13443-1, 15/26000-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MUÑOZ, Vitor Rosetto. The role of rock protein on glucose uptake in the skeletal muscle of exercised rodents during aging process. 2018. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.