MAIN BACTERIAL GROUPS FROM COLORECTAL CANCER PATIENTS´S INTESTINAL TRACT

• Grant number: 15/23666-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2016
• End date: May 31, 2017
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Mario Júlio Ávila-Campos
• Grantee: Rosa Liliana Solis Castro
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Cancer or neoplasia has an importance worldwide, and due to the high number of death, and the increase of clinical cases, this disease is considered a public health problem. In Brazil, cancer is the second leading cause of death and colorectal cancer is the most common gastrointestinal malignancy, and its incidence is increasing. Although recently there have been reported some relationship between cancer and microorganisms, no convincing scientific evidence of that microbial interaction with this process has been observed. In this study, the occurrence of different pathotypes of Escherichia coli, as well as the molecular typing of these strains by the ERIC-PCR will be evaluated. In addition, a quantitative analysis of Enterobacteriaceae, Bifidobacterium, Lactobacillus, Bacteroides spp., Clostridium Cluster I, and Phyla Firmicutes and Bacteroidetes will also be determined. Stool samples from patients with colon cancer and/or rectum, and from individuals without polyps and/or tumor, will be used as control. Other molecular techniques such as polymerase chain reaction (PCR) and quantitative real-time PCR with the SYBR Green will be used.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CASTRO, Rosa Liliana Solis. Determination of the main pathotypes of Escherichia coli in patients with rectal cancer.. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.