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THE EFFECT OF CRYOTHERAPY IN THE NOCICEPTION, INFLAMMATORY CYTOKINES AND NEUROMUSCULAR SIGNALING PATHWAYS OF MICE WITH KNEE ARTHRITIS

Grant number: 15/26567-2
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): April 01, 2016
Effective date (End): July 31, 2018
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal researcher:Tania de Fatima Salvini
Grantee:Paula Aiello Tomé de Souza Castro
Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Arthritis is an inflammatory joint disorder that can take several forms such as osteoarthritis, rheumatoid arthritis, gout, etc. When it affects the knee, can be characterized by increasing mediators in inflammatory synovial fluid, pain, swelling, thickening joint, weakness and atrophy of the quadriceps muscle. Cryotherapy is a non-pharmacological intervention widely used to control pain and inflammation in musculoskeletal disorders and is also used in knee arthritis, associated with other treatments. However, there is a lack of scientific evidence regarding the effects of cryotherapy in joint inflammation. The aim of this study is to evaluate the effect of cryotherapy in induced arthritis in the knee of mice. We will evaluate the following: nociception articular levels of pro cytokines (IL-6, IL-1², TNF-±, IL-17) and anti-inflammatory (IL-4, IL-10) in synovial fluid and possible changes morphological and molecular muscle fibers in neuromuscular junctions and in the quadriceps. Four groups will be analyzed (n = 10 per group) from C57BL / 6 mice (20-25g): Control (without intervention); Cryotherapy (Cryo); Right knee mBSA-induced arthritis antigen with intra-articular injection (arthritis); Cryotherapy and arthritis (arthritis + Cryo). Five daily sessions of cryotherapy (bag with crushed ice, 20 min) will be held in the affected limb every 2 hours, between 8 and 18h, involving the entire leg (the femoral lame joint until tibio-tarsal joint ). The first session will be held on day 21, immediately after intraarticular injection and consecutively over five sessions in 22 days. At 23 days will be assessed nociception articulate and then the animals will be euthanized to collect synovial fluid and quadriceps muscle. Statistical analysis will be performed by analysis of variance one-way ANOVA followed by multiple comparisons by Tukey test.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARBOSA, GERMANNA M.; CUNHA, JONATHAN E.; RUSSO, THIAGO L.; CUNHA, THIAGO M.; CASTRO, PAULA A. T. S.; OLIVEIRA, FRANCISCO F. B.; CUNHA, FERNANDO Q.; RAMALHO, FERNANDO S.; SALVINI, TANIA F. Thirty days after anterior cruciate ligament transection is sufficient to induce signs of knee osteoarthritis in rats: pain, functional impairment, and synovial inflammation. Inflammation Research, v. 69, n. 3, p. 279-288, MAR 2020. Web of Science Citations: 0.
BARBOSA, GERMANNA MEDEIROS; CUNHA, JONATHAN EMANUEL; CUNHA, THIAGO MATTAR; MARTINHO, LIZANDRA BOTARO; TOME SOUZA CASTRO, PAULA AIELLO; BEZERRA OLIVEIRA, FRANCISCO FABIO; CUNHA, FERNANDO QUEIROZ; RAMALHO, FERNANDO SILVA; SALVINI, TANIA FATIMA. Clinical-like cryotherapy improves footprint patterns and reduces synovial inflammation in a rat model of post-traumatic knee osteoarthritis. SCIENTIFIC REPORTS, v. 9, OCT 10 2019. Web of Science Citations: 0.
CUNHA, JONATHAN EMANUEL; BARBOSA, GERMANNA MEDEIROS; TOME DE SOUZA CASTRO, PAULA AIELLO; FERREIRA LUIZ, BEATRIZ LEITE; ARCARI SILVA, ANDREZA CRISTINE; RUSSO, THIAGO LUIZ; VASILCEAC, FERNANDO AUGUSTO; CUNHA, THIAGO MATTAR; CUNHA, FERNANDO QUEIROZ; SALVINI, TANIA FATIMA. Knee osteoarthritis induces atrophy and neuromuscular junction remodeling in the quadriceps and tibialis anterior muscles of rats. SCIENTIFIC REPORTS, v. 9, APR 24 2019. Web of Science Citations: 1.

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