Telomere length and telomerase activity in chorioamniotic membranes of term pregnancies and in spontaneous preterm birth

• Grant number: 15/26117-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2016
• End date: April 30, 2017
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Jossimara Polettini
• Grantee: Brunna Nepomuceno Colatto
• Host Institution: Faculdade de Ciências da Saúde (FCSA). Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

Premature Rupture of Membranes (PROM) is an obstetric complication characterized by disruption of the water bag before the onset of labor and occurs in approximately 40 to 50% of the pre-term labor cases, i.e. before 37 weeks of gestation. In addition to the amniotic cavity infection, recent studies show that oxidative stress inducers can activate the inflammatory cascade that triggers preterm PROM (PPROM) due to DNA damage with consequent cellular senescence. The telomere region is more vulnerable to oxidative stress and its shortening is considered a marker for cellular aging. Objective: To correlate telomere length and telomerase activity in chorioamniotic membranes from term pregnancies and from spontaneous preterm birth. Casuistic and methods: This is a retrospective study, in which chorioamniotic membranes from previous study will be included (n = 20/group). The samples are stored at Botucatu Medical School, UNESP. At the time of the gestation resolution, membrane fragments were collected in sterile conditions, immediately frozen and transported in liquid nitrogen and stored at -80°C. The demographic and obstetrical data were obtained by analysis of the medical records. The samples will be analyzed regarding telomere length by using real time PCR relative quantification (T/S). Telomerase activity will be analyzed by using commercial reagents (TRAPeze Telomerase Detection Kit ®). All pregnant women were informed about the use of samples in future studies and signed an informed consent. Statistical analysis will be performed in accordance with the conditions determined by the results and the significance level adopted will be 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COLATTO, BRUNNA NEPOMUCENO; DE SOUZA, ISABELA FIORENTINO; ANTONIO SCHINKE, LAURA APARECIDA; NODA-NICOLAU, NATHALIA MAYUMI; SILVA, MARCIA GUIMARAES; MORCELI, GLILCIANE; MENON, RAMKUMAR; POLETTINI, JOSSIMARA. Telomere Length and Telomerase Activity in Foetal Membranes from Term and Spontaneous Preterm Births. REPRODUCTIVE SCIENCES, v. 27, n. 1, p. 411-417, JAN 2020. (15/26117-7)

POLETTINI, JOSSIMARA; DA SILVA, MARCIA GUIMARAES. Telomere-Related Disorders in Fetal Membranes Associated With Birth and Adverse Pregnancy Outcomes. FRONTIERS IN PHYSIOLOGY, v. 11, p. 9-pg., 2020-10-02. (15/26117-7, 19/17279-4)