Influence of alendronate, photodynamic therapy or their combination as adjuncts to scaling and root planning for the treatment of experimental periodontitis: immunohistochemical study in rats

• Grant number: 16/04163-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2016
• End date: June 30, 2017
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Carolina dos Santos Santinoni
• Grantee: Marcela Lucio Caldeira
• Host Institution: Faculdade de Ciências da Saúde (FCSA). Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

The purpose of this study is to evaluate the effect of alendronate (ALN), photodynamic therapy (aPDT), or their combination to scaling and root planning (SRP) for the treatment of experimental periodontitis (EP) in rats. Ninety six rats will be subject to the induction of periodontitis via the installation of a ligature around the left mandibular first molar. After 7 days, the ligature will be removed, and the animals will be distributed into the following groups: SRP: SRP plus saline solution; ALN: SRP plus ALN (10-5 M); aPDT: SRP plus methylene blue irrigation followed by LLLT; ALN/aPDT: SRP plus ALN and methylene blue irrigation followed by LLLT. Each group will be divided into three subgroups (n = 8) for euthanasia at 7, 15 or 30 days after the procedures. After the routine laboratory processing, the left molar will be embedded in paraffin and 4 ¼m thick semi-serial sections will be obtained in the mesio-distal direction. The sections will be subjected to the indirect immunoperoxidase method. Receptor activator of nuclear factor- k B (RANKL) and osteoprotegerin (OPG) will be evaluate. Data will be analyzed statistically.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTINONI, CAROLINA S.; SILVEIRA, FELIPE M.; CALDEIRA, MARCELA L.; GENARO, VITOR; MARTINS, THIAGO M.; DO AMARAL, CARIL C. F.; MAIA, LUCIANA P.; MORI, GRAZIELA G.; ERVOLINO, EDILSON; POLA, NATALIA M.. Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats. JOURNAL OF PERIODONTAL RESEARCH, v. 55, n. 6, JUL 2020. (16/04163-0)