Expression of genes Cry2Aa and Cry2Ab of Bacillus thuringiensis T01 328 and evaluation of toxicity to larvae of Aedes aegypti (Diptera: Culicidae)

• Grant number: 16/03108-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2016
• End date: December 31, 2016
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Janete Apparecida Desidério
• Grantee: Marina Cabral Ricoldi
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The Bacillus thuringiensis (Bti) bacterium has a toxic activity to insects of the orders Diptera, Coleoptera and Lepidoptera. The Bti has been employed in the dengue control, a disease caused by a virus, which has become a major public health problem not only in Brazil but also worldwide. Especially in tropical countries, which favor the proliferation and development of its principal vector, the Aedes aegypti flies'. Recently, in Brazil, A. aegypti has been identified as the main vector of Chikungunya and Zika fever virus. These diseases controls are mainly carried out by combating its vector, and because Bti presents potential in the control of Diptera order insects, it is proposed in this paper to study the Cry2Aa and Cry2Ab proteins Bt T01 328 strain on biological control of A. aegypti. For comparison with the Bti strain, bioassays are performed with A. aegypti larvae with the total content of proteins extracted from Bt strains, as well as Cry2Aa and Cry2Ab proteins purified from the expression of heterologous genes in E. coli isolated from the Bt T01 328 strain. Bioassays will be performed with these proteins against A. aegypti larvae in order to estimate the LC50 and LC90. The results may suggest as an alternative the use of Bt T01 328 in vector control and contribute to management of the possible emergence of resistance to products based on Bti for A. aegypti control.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARINA CABRAL RICOLDI; CAMILA SOARES FIGUEIREDO; JANETE APPARECIDA DESIDÉRIO. Toxicidade das proteínas Cry2 do Bacillus thuringiensis subsp. thuringiensis T01-328 contra Aedes aegypti (Diptera: Culicidae). Arq. Inst. Biol., v. 85, 2018-11-23. (16/03108-5)