Time course of acute metabolic response to aerobic exercise: a metabolomics approach

• Grant number: 15/26717-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2016
• End date: March 15, 2017
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Mara Patricia Traina Chacon Mikahil
• Grantee: Marcel Lopes dos Santos
• Host Institution: Faculdade de Educação Física (FEF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Systemic metabolic responses are observed after a single aerobic training (AT) session. These responses reflect changes in physiological and biochemical processes due to physical exercise and their comprehension can contribute to develop more efficient training strategies, so as for individual fitness condition. Metabolomics, a broad technique utilized for quantitative measures of the dynamic metabolic response to a determined stimulus or due to genetic modifications in live systems, has been widely suggested for investigation and a better understanding of this response. Although, a metabolic response pattern throughout the time, from a broad perspective as the metabolomics is yet not previously reported and comparisons among studies as well as a post-exercise optimal time for biological sample collection are hard to be made. Thus, the goal of this study is to investigate the metabolic response after a single AT session in different periods from a metabolomics approach. Fifteen recreationally active healthy men, age 18-29, will be recruited for the purposes of this study. Subjects will underwent two experimental sessions in a crossover design: control session (CO) and aerobic training (AT). Sessions will occur randomly divided by a 48-hour interval. During the AT, session subjects will perform 30 minutes of cycling in a cycle ergometer with 70% of the maximum oxygen uptake (VO2max). Subjects will be kept at rest for 30 minutes during the CO session. Blood samples will be collected immediately before (-0 min) and after 0, 15, 30, 60, and 180 minutes following the 30-minute cycling for AT group and 30-minute resting for CO group. Furthermore, serum of each sample will be obtained for further analysis through nuclear magnetic resonance of protons (NMR 1H) and quantification of circulating metabolites. Multivariate statistical tests will be done for data analysis and processing. Principal component analysis (PCA) will be realized in order to identify the moment in which there is a greater metabolome (array of all identifiable metabolites) expression, and partial least squares discriminant analysis (PLS-DA) to identify which metabolites explain variations in metabolome. In addition, univariate statistical analysis will be performed for metabolites comparison between and during sessions of AT and CO (P < 0.05).