Inflammatory reaction and VEGF immunoexpression promoted by MTA Fillapex in rat tibiae

• Grant number: 16/07140-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2016
• End date: June 30, 2017
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Paulo Sergio Cerri
• Grantee: Karina Borges Salomão
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Endodontic canal treatment depends on an adequate instrumentation, an effective disinfection and a satisfactory filling procedure. Endodontic sealers are essential to obtain an adequate root canal filling and, consequently, to avoid the reinfection of the root canal. The root canal sealers may not cause tissue damage nor induce long term inflammatory reaction in the tissue supporting teeth. Moreover, these sealer materials may be biocompatible and promote the regeneration of the periodontal tissues after endodontic treatment of teeth with periapical lesions. The purpose of this study is to evaluate the tissue reaction promoted by MTA Fillapex in rat tibiae; this response will be compared with MTA, AH Plus (gold standard) and Fill Canal (negative control). Morphological features, inflammatory reaction as well as VEGF immunoexpression promoted by these materials in the bone cavities will be evaluated. Eighty adult male rats will be distributed into four groups according to the materials evaluated: MTA Fillapex (Angelus, Londrina, PR - Brazil), MTA-White (Angelus, Londrina, PR - Brazil), AH Plus (Dentsply, De Trey, Switzerland) or Fill Canal (Technew, Rio de Janeiro, RJ - Brazil). The rats will be anaesthetized with intraperitoneal injection of ketamine (80 mg/kg of body weight) combined with xylazine (4 mg/kg of body weight), the bone cavity will be prepared on the left tibia and filled with one these materials. After 7, 15, 30 and 60 days, the rats will be euthanized, the tibia fragment will be remove and fixed in formaldehyde. After the decalcification in 7% EDTA, the fragments will be embedded in paraffin. HE-stained sections will be used to morphological analysis and the numerical density of inflammatory cells will be carried out in the periosteum adjacent to the cavities. Sections will also be subjected to the immunohistochemistry reaction for VEGF detection and the numerical density of immunolabeled cells will be estimated in the periosteum. Statistical analyses will be performed using SIGMA STAT 2.0 (Jandel Scientific, Sausalito, CA, USA); the data will be subjected to ANOVA and Tukey tests. The significance level accepted will be p£0.05.