Physiological responses, bioactivity and analysis of protein differential expression of the brown alga Sargassum sp. C. Agardh (Phaeophyceae, Fucales) submitted to UV radiation

• Grant number: 16/03095-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2016
• End date: September 30, 2019
• Field of knowledge: Biological Sciences - Botany - Applied Botany
• Principal Investigator: Fanly Fungyi Chow Ho
• Grantee: Luz Karime Polo Osorio
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/17758-7 - Impact of UV radiation on chemical composition of Sargassum sp.: chlorophyll, carotenoids and fatty acids profiles, BE.EP.DR

Abstract

Aquatic ecosystems are the main components of earth and produce more than 50% of planet biomass. Within this ecosystem, algae contribute significantly to global biodiversity, performing one or more functions and ecosystem services. Ecophysiological responses and the potential use and exploitation of macroalgae are influenced by environmental conditions as UV radiation. UV radiation effects studies are essential to understanding the metabolic basis of physiological adaptation of macroalgae and can support the understanding of the possible effects of global climate change by significant impact on distribution patterns, vertical zonation and primary productivity. Based on the aforementioned information, the answers brown seaweed Sargassum sp. will be studied to determine the effect produced by UV radiation in the different biological and physiological processes. Aspects as such as growth rate, pigment content, antioxidant potential and biological activity as well as its differential expression of proteins after treatment with UV radiation (PAR, PAR + UVA and UVB + PAR) will be assessed. The monitoring of the various responses will be analyzed at four different times (0, 4, 7 and 10 days); however, the analysis of antioxidant activity, bioactive potential, and protein differential expression will be evaluated in two stages (0 and 10 days). This study will help answer whether the UVA and UVB radiation differently affect the biological and physiological processes in Sargassum sp., and if there is differential expression of certain proteins and changes in the bioactivity under these conditions.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

POLO, LUZ K.; CHOW, FUNGYI. Physiological performance by growth rate, pigment and protein content of the brown seaweed Sargassum filipendula (Ochrophyta: Fucales) induced by moderate UV radiation exposure in the laboratory. Scientia Marina, v. 84, n. 1, p. 59-70, MAR 2020. (16/03095-0, 13/50731-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OSORIO, Luz Karime Polo. Physiological responses, bioactivity and differential analysis of protein abundance of the brown seaweed Sargassum (Phaeophyceae, Fucales) submitted to UV radiation. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.