Analysis of the microarchitecture and dynamics of intramembranous and endochondral bones of rats treated with intravenous bisphosphonate

• Grant number: 16/03448-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2016
• End date: June 30, 2017
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Mariza Akemi Matsumoto
• Grantee: Flávio Duarte Faria
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Prolonged and wide administration of the bisphosphonates-containing nitrogen for prevention and treatment of osteolitic diseases have made possible the identification of the adverse effects they provoke at systemic level and in the skeleton by various methodologies. The aim of the present study is to analyze and to compare microarchitecture characteristics of intramembranous (maxillae and mandible) and endochondral (femur), as well as the remodeling dynamics under the influence of zoledronic acid. Fourteen male Wistar rats, age of 12 months, will be distributed into two groups, according to the treatment: Control - 0.1 ml of intravenous (IV) 0.9% saline solution, and ZL - 0.035 ml/Kg of IV zoledronic acid. All animals of both groups will be treated every 15 days, and the substances will be administered in the tail vein. After the fourth administration of the substances, all the animals will receive intramuscular (IM) injection of the fluorochrome calcein, in the dose of 20 mg/Kg for each animal. After 14 days of calcein injection, 20 mg/Kg of fluorochrome of alizarin will be administered, via IM. After 14 days of alizarin injections, all the animals will be euthanized for the removal of the maxillae, mandible and femur. The specimens will be immediately fixed in 10% buffered formalin for 48 hours, washed in tap water for 24 hours, and immersed in 70% alcohol to be scanned in micro-computed tomograph (microCT). After this procedure, the specimens will undergo dehydration process for resin inclusion, in order to be prepared for the analysis of bone remodeling under epifluorescence.