Characterization of the mechanisms underlying omega-3 fatty acids effects on energy balance

• Grant number: 16/07062-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2016
• End date: December 31, 2017
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: William Tadeu Lara Festuccia
• Grantee: Tiago Eugênio Oliveira da Silva
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/19530-5 - Involvement of the nutrient sensor mTOR in the development of obesity associated chronic metabolic diseases, AP.TEM

Abstract

Recent studies from our laboratory have shown that increased body content of omega 3 (n-3) fatty acids, through the intake of a diet rich in fish oil or expression of fat-1 desaturase which converts omega 6 to n-3, protect mice from diet induced obesity, inflammation and glucose intolerance. Interestingly, such protection from obesity induced by n-3 fatty acids occur independently of changes in food intake, indicating a likely involvement of energy expenditure as a underlying cause. Herein we will test the hypothesis that n-3 fatty acids enhance energy expenditure in mice through the modulation of uncoupling protein-1 (UCP-1) dependent and independent thermogenesis. To test this hypothesis we will perform two different protocols: first we will assess the effects of different concentrations of n-3 fatty acids on C57BL/6J mice body weight, energy balance, adiposity, energy expenditure, sympathetic activity to brown and white adipose tissue, mitochondrial content and function, hypothalamus-pituitary-thyroid status, body temperature, and brown and white adipose tissues morphology, contents of UCP1, deiodinase type 2, SERCA 1 e 2, and other thermogenesis- related proteins. In the second protocol, we will evaluated the same parameters in wild-type and UCP-1 deficient mice fed with a regular high-fat diet or a fish oil enriched diet to investigate the contribution of nonshivering thermogenesis to the increase in energy expenditure induced by n-3 fatty acids. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASTRO, ERIQUE; SILVA, TIAGO E. OLIVEIRA; FESTUCCIA, WILLIAM T.. Critical review of beige adipocyte thermogenic activation and contribution to whole-body energy expenditure. HORMONE MOLECULAR BIOLOGY AND CLINICAL INVESTIGATION, v. 31, n. 2, SI, AUG 2017. (16/23169-9, 16/07062-0, 15/19530-5)

CASTRO, ERIQUE; SILVA, TIAGO E. OLIVEIRA; FESTUCCIA, WILLIAM T.. Critical review of beige adipocyte thermogenic activation and contribution to whole-body energy expenditure. HORMONE MOLECULAR BIOLOGY AND CLINICAL INVESTIGATION, v. 31, n. 2, p. 9-pg., 2017-08-01. (15/19530-5, 16/23169-9, 16/07062-0)

BLANCHARD, PIERRE-GILLES; MOREIRA, RAFAEL J.; CASTRO, ERIQUE; CARON, ALEXANDRE; COTE, MARIE; ANDRADE, MAYNARA L.; OLIVEIRA, TIAGO E.; ORTIZ-SILVA, MILENE; PEIXOTO, ALBERT S.; DIAS, FRANCE ANNE; et al. PPAR gamma is a major regulator of branched-chain amino acid blood levels and catabolism in white and brown adipose tissues. METABOLISM-CLINICAL AND EXPERIMENTAL, v. 89, p. 27-38, DEC 2018. (15/19530-5, 16/07062-0, 17/12260-8, 16/23169-9, 15/13508-8)

OLIVEIRA, TIAGO E.; CASTRO, ERIQUE; BELCHIOR, THIAGO; ANDRADE, MAYNARA L.; CHAVES-FILHO, ADRIANO B.; PEIXOTO, ALBERT S.; MORENO, MAYARA F.; ORTIZ-SILVA, MILENE; MOREIRA, RAFAEL J.; INAGUE, ALEX; et al. Fish Oil Protects Wild Type and Uncoupling Protein 1-Deficient Mice from Obesity and Glucose Intolerance by Increasing Energy Expenditure. MOLECULAR NUTRITION & FOOD RESEARCH, v. 63, n. 7, APR 2019. (15/19530-5, 17/17582-3, 16/07062-0, 17/12260-8, 15/22983-1, 16/23169-9, 15/13508-8, 18/11156-5, 17/23040-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Tiago Eugênio Oliveira da. Intake of diet enriched fish oil increases energy expenditure by a UCP1-independent mechanism and protects mice from obesity induced by lard-rich diet.. 2018. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.