Study of differential gene expression induced by Porcine Circovirus type 2 in swine testicle cells and piglets experimentally infected

• Grant number: 16/09518-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2016
• End date: November 09, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: João Pessoa Araújo Junior
• Grantee: Taís Fukuta da Cruz
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 17/26649-4 - Impact of a new porcine circovirus (PCV3) infection: diagnostic tools development and disease association, BE.EP.PD

Abstract

Porcine circovirus type 2 (PCV2) are small, icosahedral, non-enveloped viruses containing a circular single-strand DNA genome, noncytopathogenic, which belong to the family Circoviridae. PCV2 is the causal agent of Post-weaning multisystemic wasting syndrome (PMWS), a great economic important disease worldwide distributed. The viral isolation of PCV2 in cell culture is difficult, laborious, and produces low viral titres. However, the preparation of cell cultures of swine testicle (ST) persistently infected with PCV2b, cultivated without performing passages, with high viral loads, and producing cytopathic effect, it was reported by our research group. As PCV2 infection is characterized by being persistent and causing severe immunosuppression, there is the need for more research to elucidate this important aspect of the pathogenesis of the disease. Therefore, the objective of this project is to perform the study of the transcriptional profile of persistently infected ST cells compared to the control cells, and animals experimentally infected with genotypes PCV2a, 2b, and 2c using RNA-seq technique (RNA sequencing). Then the genes that were differentially expressed will be analyzed by RT-qPCR "in vitro" (ST cells) and "in vivo" (animals natural and experimentally infected with PCV2). In addition, the next generation sequencing (NGS) will be used to sequence the entire viral genome of samples analyzed in this project. Thus, this research will be able provide new information regarding the pathogenesis of the disease, aiming to help in the prevention of this important viral disease. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TANIWAKI, SUELI A.; JIMENEZ-VILLEGAS, TATIANA; SANTANA-CLAVIJO, NELSON F.; CRUZ, TAIS F.; SILVA, SHEILA O. S.; VALENCIA-BACCA, JUAN D.; LOAIZA-PEDREROS, FELIPE; RICHTZENHAIN, LEONARDO J.; FERREIRA, FERNANDO; ARAUJO JUNIOR, JOAO P.; et al. Near-Complete Genome Sequence of Feline Immunodeficiency Virus from Colombia. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 9, n. 33, AUG 2020. (16/09518-0, 17/24907-6)

DAROLD, GABRIELA MOLINARI; ALFIERI, AMAURI ALCINDO; ARAUJO JUNIOR, JOAO PESSOA; DA CRUZ, TAIS FUKUTA; BERTTI, KARYTA MARIA DE LIMA BEZERRA; DA SILVA, GLAUCENYRA CECILIA PINHEIRO; AMUDE, ALEXANDRE MENDES; MURARO, LIVIA SAAB; LAVORENTE, FERNANDA LOUISE PEREIRA; LUNARDI, MICHELE. High genetic diversity of paramyxoviruses infecting domestic cats in Western Brazil. TRANSBOUNDARY AND EMERGING DISEASES, DEC 2020. (16/09518-0)

TAÍS FUKUTA CRUZ; THIAGO NEVES BATISTA; ESTER MARIANE VIEIRA; LUIZ MARCOS FREDIANI PORTELA; AMANDA MAHNKE BACCARIN; JÉSSICA JEHLE GRADIZ; JOÃO PESSOA ARAÚJO JUNIOR. Caracterização genômica do Canine circovirus detectado em um cão com gastroenterite hemorrágica intermitente no Brasil. Ciência Rural, v. 50, n. 5, 2020-04-27. (16/09518-0)

DAROLD, GABRIELA MOLINARI; ALFIERI, AMAURI ALCINDO; ARAUJO JUNIOR, JOAO PESSOA; DA CRUZ, TAIS FUKUTA; BERTTI, KARYTA MARIA DE LIMA BEZERRA; DA SILVA, GLAUCENYRA CECILIA PINHEIRO; AMUDE, ALEXANDRE MENDES; MURARO, LIVIA SAAB; LAVORENTE, FERNANDA LOUISE PEREIRA; LUNARDI, MICHELE. High genetic diversity of paramyxoviruses infecting domestic cats in Western Brazil. TRANSBOUNDARY AND EMERGING DISEASES, v. 68, n. 6, p. 3453-3462, NOV 2021. (16/09518-0)