CHEMOKINES AND FIBRINOLYSIS EVALUATION IN EQUINES SUBMITTED TO ANASTOMOSIS OF DESCENDING COLON UNDERGOING BIOMATERIAL APLICATION OR NOT

• Grant number: 16/09713-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2016
• End date: January 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Áureo Evangelista Santana
• Grantee: Helena Cristina Delgado Brito
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Intra-abdominal adhesions are a common complication in equine gastro-intestinal tract surgery, being originated by inflammatory processes in abdominal cavity. Adhesions usually results from the disbalance of fibrinolysis inhibitors and activators, which are activated by several chemokines. The aim of this study is to evaluate and quantify fibrinolysis markers as tissue plasminogen activator (tPA) and D-dimers, and also the genic expression of interleukins 1 (IL-1), IL-10, IL-17 and correlate this findings with the development of intestinal adhesions after descending colon anastomosis of equine undergoing biomaterial application or not. Twenty four healthy equines, males or females, with no defined breed, aging between 4 and 15 years divided into four experimental groups, containing six animals per group. All animals will be subjected to ventral midline celiotomy in dorsal decubitus under general inhalatory anesthesia for descendent colon end-to-end anastomosis. After completion of anastomosis in the animals from control group (CG) the intestinal area sutured will only be washed with physiological solution; in treated group 1 (TG1) the abdominal cavity will be infused with streptokinasis; in treated group 2 (TG2), a biomaterial without streptokinasis will be implanted in descendent colon serous; while in treat group 3 (TG3) a biomaterial containing streptokinasis will be implanted in the same intestinal serous region. Clinical parameters of all experimental animals will be evaluated on a daily basis, during 14 days. In the 14th day post anastomosis, a videolaparoscopy will be accomplished to observe healing process on the descending colon and therefore the development of intra-abdhominal abscess, open dehiscence, adhesions and abdominal wall or inguinal infection. Venous jugular blood and peritoneal fluid samples will be collected for laboratorial analysis during immediate preoperative period regarding to the anastomosis and postoperative. Data obtained from the studied variables will be tabulated, statistically analised, interpreted and described as a PhD thesis and published in arbitrary journals.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BRITO, Helena Cristina Delgado. Clinical and laboratorial evaluation in horses subjected to enterotomy of descending colon undergoing biomaterial aplication. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.