Clinical and laboratorial evaluation in horses subjected to enterotomy of descending colon undergoing biomaterial aplication

Author(s)

Helena Cristina Delgado Brito

• Document type: Doctoral Thesis
• Press: Jaboticabal. 2019-05-10.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2019-02-01
• Advisor: Aureo Evangelista Santana; Letícia Abrahão Anai

Abstract

Intra-abdominal adhesions are a complication after gastrointestinal tract surgery in equines, originated by inflammatory processes in the abdominal cavity. Adhesions usually result from the imbalance between fibrinolysis inhibitors and activators, which are activated by several chemokines. The aim of this study is to evaluate the clinical and laboratorial alterations and observe the development of intestinal adhesions after descending colon enterotomy of equine undergoing application or not of biomaterial composed by carboxymethyl cellulose (CMC) and polyvinil alcohol (PVA) or CMC and PVA impregnated with alteplase. Sixteen healthy crossbred mares, mixed breed, aged between 4 and 15 years randomly allotted in 4 groups: control group (CG), treated group 1 (TG1), which received abdominal alteplase infusion, treated group 2 (TG2), which received a biomaterial implant on the colon serosa and treated group 3 (TG3), which received the biomaterial containing alteplase implant on the colon serosa, were used in this study. All animals were subjected to left flank laparotomy and descending colon enterotomy. Clinical parameters were evaluated daily for 12 days (M0 – M12). On the 12th day postsurgery, videolaparoscopy was performed to evaluate whether there was of not abdominal adherences. Venous jugular blood and peritoneal fluid samples were collected for laboratorial analysis during immediate preoperative period (M0) and 1 (M1), 3 (M3), 5 (M5), 7 (M7), 9 (M9) and 11 (M11) days after the enterotomy. In the clinical parameters, rectal temperature decreased in GT3 in M5 and M12. Intestinal motility decreased in the first three days post-surgery (M0, M1 and M3) for all groups. Hematocrit values decreased in M1 when compared to M0, and GT3 presented the lower value when compared to GC and GT1. Equines presented neutrofilia, lymphopenia and eosinophenia in M1, followed by leukopenia in M3 and M5. Peritoneal fluid analysis revealed reduction in pH values and glucose concentration in M1, and an increase in lactate concentration and total protein in M1. Nucleated cells total count and neutrophil counts in peritoneal fluid increased in all moments when compared to M0. Abdominal adhesions were present in 50% of GC, GT1 and GT2 groups, and 100% in GT3. Alteplase infusion, biomaterial, and biomaterial containing alteplase did not produce satisfactory results regarding preventing intestinal adhesions in horses. (AU)

• FAPESP's process: 16/09713-8 - CHEMOKINES AND FIBRINOLYSIS EVALUATION IN EQUINES SUBMITTED TO ANASTOMOSIS OF DESCENDING COLON UNDERGOING BIOMATERIAL APLICATION OR NOT
• Grantee: Helena Cristina Delgado Brito
• Support Opportunities: Scholarships in Brazil - Doctorate