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Equine descending colon healing potential analysis through videolaparoscopy, histomorphometry and dosage of hydroxyproline after experimental obstruction: clinical, pathological and laboratorial aspects

Abstract

The colic syndrome is a painful process of the abdominal cavity and is a major cause of emergency surgery in veterinary medicine. The equine digestive system is the site of important clinical disorders that lead these animals to death. The aim of this study is to evaluate the healing potential of experimentally induced ischemic lesions in the descending colon of horses by histological and morphometric analysis and determination of intestinal tissue concentration of hydroxyproline, correlating to the possible clinical changes. This study uses eight healthy mixed-breed horses, of both gender, aged between five and eight years. The animals are subjected to laparotomy and experimental intraluminal obstruction of the descending colon for 240 minutes. Biopsies of the descending colon will be made before and after the obstruction and seven days after. Twenty-one days after experimental obstruction videolaparoscopy is performed to evaluate the healing process. After processing the biopsy material, it will be stained with hematoxylin-eosin (HE), Masson's trichrome and picrosirius red. Histological and morphometric analysis be carried out in order to qualify and quantify the inflammatory infiltrate, measure the thickness of the submucosal layer and verify and quantify the degree of intermingling of collagen fibers. Quantification of intestinal tissue collagen will also be determined by the relationship between the concentrations of hydroxyproline and total protein tissue, using the spectrophotometric method. The study will be complemented with clinical evaluations of the animals and the data assessed through statistical analysis. (AU)

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