Clinical, laboratorial and surgical aspects of equine anastomosis of descending colon undergoing biomaterial aplication or not

• Grant number: 15/17504-7
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2015
• End date: November 30, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Áureo Evangelista Santana
• Grantee: Áureo Evangelista Santana
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Intra-abdominal adhesions are a common complication in equine gastro-intestinal tract surgery, leading to obstructive processes, abdominal pain and intestinal volvulus. The aim of this study is to produce and evaluate the laboratorial and in vivo efficiency of biomaterial when applied as graft to avoid the development of intestinal adhesions after descending colon anastomosis. For in vivo validation, initially, two experimental groups of six Wistar rats each, being: biomaterial without streptokinasis (GD1) and biomaterial containing streptokinasis (GD2). All rats will be subjected to ventral midline celiotomy, descendent colon end-to-end anastomosis and euthanasia after 14 days for macroscopic evaluation of the healing process on the descending colon (development or not of adhesions, abdominal infection and open dehiscence). After the biomaterial validation, four experimental groups of six equines (24 animals) will be subjected to the same anastomosis technique cited above, being: equines submitted to a wash with physiological solution in the intestinal area sutured (CG); abdominal infusion of streptokinasis (TG1); an implant of biomaterial without streptokinasis in the anastomosis área (TG2) and an implant of biomaterial containing streptokinasis in the anastomosis área (TG3). Equine clinical parameters will be evaluated daily, during 14 days. On the 14th day, videolaparoscopy will be accomplished to evaluate the same healing parameters described in murine model. Equine venous jugular blood and peritoneal fluid samples will be collected for laboratorial analysis during immediate preoperative, transoperative period regarding to the anastomosis and postoperative. Solely in equine's TG1 and TG3 serial blood samples will be collected for streptokinasis pharmacokinetics analysis. Data obtained from clinical, laboratorial and cirurgical variables will be evaluated by statistical analysis. (AU)