Morphological and ultrastructural evaluation of the equine descending colon and epidermal/dermal tissue after the challenge due to obstruction do not throttle intraluminal descending colon

• Grant number: 11/22501-6
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2012 - May 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: José Corrêa de Lacerda Neto
• Grantee: José Corrêa de Lacerda Neto
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Colic syndrome is a major cause of emergency surgery in veterinary medicine. The equine digestive system is a source of important clinical disorders that lead these animals to death, if not, may cause laminitis, another extremely painful and debilitating disorder in which there is separation of dermal and epidermal sheets responsible for supporting the wall of the distal phalanx of the hull, well-recognized and serious complication that not uncommonly manifests itself during or after episodes of acute abdomen. Despite the importance of the occurrence of colic caused by changes involving the descending colon, there are a few experimental studies focusing on this portion of the intestinal tube. The aim of this study is to analyze in ultrastructural way cells of the descending colon, and skin tissue of horses foot during experimentally induced ischemia by intraluminal obstruction of the descending colon. Eight healthy adult horses, male or female, mixed breed, between five and 15 years old will be used. The animals will undergo initially celiotomy and intraluminal obstruction of the descending colon with a duration of 240 minutes. Biopsy of segments of the wall of the descending colon will be performed, before the end of the obstruction, as well as the foot skin tissue. After processing, the biopsy material will be analyzed by transmission electron microscopy to obtain images which will be subsequently submitted to analysis. The analysis will be performed in order to evaluate ultraestrutral morphological changes that occurred in the cells of the descending colon as well as the dermal tissue / epidermal skin or foot in these horses. The data obtained will be evaluated by statistical analysis. (AU)