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Identification of priority areas for the conservation of threatened species in the Mogi-Guaçu river using techniques of DNA barcode

Grant number: 15/19025-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): August 01, 2016
Effective date (End): October 31, 2019
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
Principal researcher:Claudio de Oliveira
Grantee:André Batista Nobile
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated scholarship(s):17/12758-6 - Use of DNA metabarcoding as alternative, resolute and low-cost tool to DNA barcoding in ecological studies, BE.EP.PD


The neotropical freshwater fish fauna is the most diverse existing in the world, although this expressive diversity of fish is threatened by several human impacts. Among these, the impoundment of rivers is one of the most serious threats, especially to rheophilics species that require large free passages to carry out their reproductive cycle and use floodplains and lagoons as breeding sites and nursery areas. Studies involving the ichthyoplankton have been an important tool in identifying breeding sites, however, the traditional identification of eggs and larvae presents serious limitations, often not allowing to identification below the family level. The molecular identification of species using a short segment of the gene mitochondrial Cytochrome C oxidase subunit I (COI), called DNA barcoding proved extremely effective in the identification of eggs and larvae, both in marine environments such as freshwater environments. Thus, the aim of this project is to use this molecular tool to identify the ichthyoplankton, enabling the detection of reproductive and nursery areas sites in a lowland area of the Mogi-Guaçu.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA COSTA, GABRIELA O.; NOBILE, ANDRE B.; LIMA, FELIPE P.; FREITAS-SOUZA, DIOGO; MANZANO, FELIPE V.; OLIVEIRA, CLAUDIO. Length-weight and length-length relationships for four fish species of Upper Paranaiba river basin, Brazil. Journal of Applied Ichthyology, v. 35, n. 2, p. 619-621, APR 2019. Web of Science Citations: 0.
AZEVEDO-SANTOS, V. M.; COELHO, P. N.; BRAMBILLA, E. M.; LIMA, F. P.; NOBILE, A. B.; BRITTON, J. R. Length-weight relationships of four fish species from the upper Parana River basin, Southeastern Brazil. Journal of Applied Ichthyology, v. 34, n. 1, p. 237-239, FEB 2018. Web of Science Citations: 4.
NOBILE, A. B.; FREITAS-SOUZA, D.; LIMA, F. P.; VIEIRA, L. B.; MELO, B. F.; OLIVEIRA, C. Length-weight and length-length relationships of 16 fish species from Amapa, Brazilian Amazon. Journal of Applied Ichthyology, v. 33, n. 5, p. 1058-1061, OCT 2017. Web of Science Citations: 5.

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