Co-immobilization of enzymes acetylcholinesterase and beta-secretase 1: study of conditions for screening of ligands

• Grant number: 16/15037-5
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): October 01, 2016
• Effective date (End): July 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal researcher: Carmen Lúcia Cardoso
• Grantee: Adriana Ferreira Lopes Vilela
• Supervisor abroad: Cesar Mateo
• Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Research place: Instituto de Catálisis y Petroleoquímica (ICP), Spain
• Associated to the scholarship: 16/02873-0 - Acetylcholinesterase and beta-secretase 1: a study of co-immobilization conditions for screening of ligands, BP.DR

Abstract

Enzymes have excellent properties as activity, selectivity and specificity, which ensures its high application potential, but are limited due to the high cost and low stability in solution. With advances in technology and in an attempt to take advantage of all the catalytic properties of enzymes for longer, immobilization methods are an alternative to in vitro studies. If there is more than one target of interest, co-immobilization of enzymes may also be applied. This is a technique that involves the immobilization of two or more targets on the same support and these simultaneously used during screening ligands. In this case have to creation of a specific environment that mimics a cell may be considered as models of compartmentalized intracellular enzymes and in vitro models for cellular processes. The enzymes cholinesterases and secretases are targets of interest in drug development studies for Alzheimer's disease, since they are involved in the etiology and progress of the disease. Considering the interest in inhibitors of these enzymes, which act in the presence of both, the objective of this project is the development of the method of co-immobilization involving enzymes acetylcholinesterase (AChE) and ²-secretase-1 and application for identifying active substances in collections natural and / or synthetic. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES DE MELO, RICARDO; CARLOS ALNOCH, ROBSON; LOPES VILELA, ADRIANA FERREIRA; DE SOUZA, EMANUEL MALTEMPI; KRIEGER, NADIA; RULLER, ROBERTO; SATO, HELIA HARUMI; MATEO, CESAR. New Heterofunctional Supports Based on Glutaraldehyde-Activation: A Tool for Enzyme Immobilization at Neutral pH. Molecules, v. 22, n. 7, JUL 2017. (16/15037-5, 13/01710-1)

LOPES VILELA, ADRIANA FERREIRA; CARDOSO, CARMEN LUCIA; MATEO, CESAR. An immobilized acetylcholinesterase as test system to screen new inhibitor drugs to treat Alzheimer's disease. SENSORS AND ACTUATORS B-CHEMICAL, v. 278, p. 196-201, JAN 1 2019. (16/15037-5, 13/01710-1)