Experimental urolithiasis model in sheep: clinical, laboratory and blood gas study

• Grant number: 16/12746-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2016
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Luiz Claudio Nogueira Mendes
• Grantee: Mariana Maria Branco Rosetti
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The high incidence of obstructive urolithiasis in sheep, occurring mainly in confined, is multifactorial and subject of several studies, it causes economic damage, affecting the reproductive health of the animal and even leading to his death. The objective of this work is to develop an experimental model of urolithiasis in sheep by ammonium chloride administration in two doses. Will be used 25 male sheep, aged approximately three months confined randomly divided into 3 groups: Group I (n = 10) 200 mg/kg/PV ammonium chloride/animal/day; Group II (n = 10) 500 mg/kg/PV ammonium chloride/animal/day & GROUP III (n = 5) control, without adding ammonium chloride, for a period of 42 days. Before the start of the experiment, all animals will be wormed and vaccinated against clostridial diseases. Will undergo a period of adjustment for 7 days to the environment, allocated in separate stalls in groups, in wet conditions, identical temperature and light, with a total period of confinement of 49 days. The analysis will be done through urinalysis, ultrasound, identifying the type of calculation and biochemical analysis using ammonium chloride (CA) and evaluating its effect on urolithiasis. Moreover, we can identify the type of urolith, frequency and obstruction sites, as well as its consequences, can the model be used in sheep and humans. Continuous variables will be analyzed by the Kruskal-Wallis test, and when there is a statistically significant difference, verified by post-hoc Dunn test. The median will be evaluated by the Friedman test and the data of urinalysis and physical examination will be analyzed by IBM SPSS Statistics Software, v21, with a significance level of 5% (p <0.05).