Cutaneous leishmaniasis biomarkers candidates search and identification through mass spectrometry

• Grant number: 16/11517-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2016
• End date: January 31, 2020
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Marcos Nogueira Eberlin
• Grantee: Fernanda Negrão Silva
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 17/22651-4 - Shotgun proteomics for host-pathogen relationship investigation of leishmaniasis in vitro and in vivo, BE.EP.DD

Abstract

Leishmaniasis, caused by protozoa of the genus Leishmania, is a neglected disease in Brazil and worldwide. This proposal aims to apply the MS in the search of biomarkers candidates of Cutaneous Leishmaniasis (CL), the most common clinical form of the disease. The project involves the imaging of proteins and lipids in lesions of infected mice (widely used in experimental models of this disease) referred to the species L. amazonensis and L. major. The MS techniques to be applied, in special MALDI (Matrix Assisted Laser Desorption Ionization) and DESI (Desorption Electrospray Ionization), allows detection of biomolecules with high sensitivity, accuracy and selectivity compared to other classical biomolecules chemical analysis techniques, such as immunohistochemistry, immunofluorescence and immunoblotting. The search of biomarkers candidates with these methodology will give more information about the pathophysiological mechanisms of Leishmania spp., providing excellent prospects in new diagnostic tests and drug development. This proposal will contribute effectively to improve the understanding of the parasite Leishmania spp. biology and pathophysiology. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NEGRAO, FERNANDA; ABANADES, DANIEL R.; JAEEGER, CAROLINE F.; ROCHA, DANIELE F. O.; BELAZ, KATIA R. A.; GIORGIO, SELMA; EBERLIN, MARCOS N.; ANGOLINI, CELIO F. F.. Lipidomic alterations of in vitro macrophage infection by L-infantum and L-amazonensis. MOLECULAR BIOSYSTEMS, v. 13, n. 11, p. 2401-2406, NOV 2017. (16/11517-2, 15/23767-0, 12/07206-0, 13/11100-6, 10/51677-2)

SAVIOLA, ANTHONY J.; NEGRAO, FERNANDA; YATES, JOHN R., III; BOHN, PW; PEMBERTON, JE. Proteomics of Select Neglected Tropical Diseases. ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 13, v. 13, p. 22-pg., 2020-01-01. (16/11517-2)

JAEGGER, C. F.; NEGRAO, F.; ASSIS, D. M.; BELAZ, K. R. A.; ANGOLINI, C. F. F.; FERNANDES, A. M. A. P.; SANTOS, V. G.; PIMENTEL, A.; ABANADES, D. R.; GIORGIO, S.; et al. MALDI MS imaging investigation of the host response to visceral leishmaniasis. MOLECULAR BIOSYSTEMS, v. 13, n. 10, p. 1946-1953, OCT 2017. (16/11517-2, 15/23767-0, 13/11100-6, 12/07206-0)

NEGRAO, FERNANDA; ROCHA, DANIELE F. DE O.; JAEEGER, CAROLINE F.; ROCHA, FRANCISCA J. S.; EBERLIN, MARCOS N.; GIORGIO, SELMA. Murine cutaneous leishmaniasis investigated by MALDI mass spectrometry imaging. MOLECULAR BIOSYSTEMS, v. 13, n. 10, p. 2036-2043, OCT 2017. (16/11517-2, 15/23767-0)

NEGRAO, FERNANDA; EBERLIN, MARCOS NOGUEIRA; GIORGIO, SELMA. Proteomic approaches for drug discovery against tegumentary leishmaniasis. BIOMEDICINE & PHARMACOTHERAPY, v. 95, p. 577-582, NOV 2017. (16/11517-2, 15/23767-0)

PORCARI, ANDREIA M.; NEGRAO, FERNANDA; TRIPODI, GUILHERME LUCAS; PITTA, DENISE ROCHA; CAMPOS, ELISABETE APARECIDA; MONTIS, DOUGLAS MUNHOZ; MARTINS, ALINE M. A.; EBERLIN, MARCOS N.; DERCHAIN, SOPHIE F. M.. Molecular Signatures of High-Grade Cervical Lesions. FRONTIERS IN ONCOLOGY, v. 8, APR 12 2018. (16/11517-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Fernanda Negrão. Application of mass spectrometry-based proteomics and lipidomics to search for molecular alterations in the host infected by Leishmania spp.. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.