Expression of accessory enzymes polysaccharide mono-oxygenase and cellobiose dehydrogenase from Aspergillus nidulans in Trichoderma reesei and evaluation of the degradation efficiency of sugar cane bagasse

• Grant number: 16/09708-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2016
• End date: May 31, 2018
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Roberto do Nascimento Silva
• Grantee: Douglas Christian Borges Lopes
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Trichoderma reesei is a main fungus used for the large scale production of several enzymes, being widely studied microorganism. This is due to the great capacity of production and secretion of cellulases and hemicellulases for use in saccharification processes of lignocellulosic biomass. This is mainly due to the great capacity of production and secretion of cellulases and hemicellulases for use in saccharification processes of lignocellulosic biomass. Because of biotechnological importance of these enzymes in the plant biomass degradation, T. reesei have been used for the production of these enzymes at low cost for use in the depolymerization of the biomass, with greater emphasis to the bioethanol production. The degradation step of the cellulosic fraction of lignocellulosic biomass, in addition to the action of cellulases, to have aware of other enzymes that help in the process, known as accessory enzymes. These enzymes, in combination with cellulases, allow an increase in cellulose depolymerization, such as polysaccharide monooxygenases (PMOs) and cellobiose dehydrogenases (CDHS). Although T. reesei is used as a major producer of cellulases at industrial scale, various processes and studies are conducted to improve the understanding of the whole plant biomass reuse mechanism allowing increased efficiency in the production and cellulases activity. Thus, the aim of this study is to express and evaluate the influence of a polysaccharide monooxygenases and cellobiose dehydrogenases, from Aspergillus nidulans in plant biomass degradation process and cellulases production by Trichoderma reesei. For this, T. reesei transformation is performed for the obtainment of transformants which express these enzymes alone and/or together, and subsequently evaluated for degradation sugarcane bagasse by enzymatic hydrolysis; assays measuring reducing sugars; high-performance liquid chromatography, and assays to measuring the viscosity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, DOUGLAS CHRISTIAN BORGES; CARRARO, CLAUDIA BATISTA; SILVA, ROBERTO NASCIMENTO; DE PAULA, RENATO GRACIANO. Molecular Characterization of Xyloglucanase cel74a from Trichoderma reesei. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 9, MAY 2021. (16/23233-9, 16/09708-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LOPES, Douglas Christian Borges. Functional characterization of Trichoderma reesei xyloglucanase (CEL74A) in degradation of sugarcane bagasse. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.