Evaluation of the effects of hyaluronic acid and poly-L-lactic acid in the synthesis of Collagen type i and inflammatory process: in vivo study

• Grant number: 16/11936-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2016
• Effective date (End): September 30, 2017
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Elizabeth Ferreira Martinez
• Grantee: Larissa Rocha Bertelli Cabral
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Aging is a complex biological process involving multifactorial dynamic interactions, with three-dimensional changes in all organs and systems. At the skin, in cellular level, due to aging, the process of synthesis and degradation of extracellular matrix has been established, however with changes in the profile and molecular composition, and as a result, there is the anatomical and clinical changes that occur over time. Thus, the dermal fillers have become widely used for correcting aging changes, as well as diseases that involves the skin. Among the materials available, we do have hyaluronic acid (HA) and poly-L-lactic acid (PLLA), both biocompatible and biodegradable, but they can cause inflammatory reaction after application, despite the low immunogenicity. Therefore, considering the high demand for use of fillers materials, this study aims to evaluate the synthesis of collagen type I and the initial inflammatory response mediated by TNF-±, IL-1 and IL-6 after intradermal injection of HA and PLLA in animal models, by Western-blotting. (AU)