Influence of scaffolds of poly(butylene adipate-co-terephthalate) embedded with carbon and nano-hydroxyapatite nanotubes in bone repair of critical defects

• Grant number: 16/04618-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2016
• End date: September 30, 2017
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Luana Marotta Reis de Vasconcellos
• Grantee: Juliani Caroline Ribeiro de Araújo
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Biodegradable scaffolds have shown great potential to aid in tissue engineering. The combination of factors such as the distribution of nanofibers and the incorporation of nanoparticles, which can guide and accelerate bone cells, can optimize the process of bone repair. In this study will be produced scaffolds PBAT embedded with carbon nanotubes nanoparticles (CNTs) and nanohidroxiapatita (nHA), to orthopedic and dental applicability. The scaffolds will be produced by electrospinning technique and divided into three groups according to their composition: a) PBAT (control); b) PBAT incorporated with nHA; c)PBAT embedded with nHA/CNTs. Sixty male adult rats will be used on this study. First of allit will be done 3 centimeters drillings in right and left tibias with serial drills and plenty irrigation. The animals will be separated in 3 groups according to the left tibia filling bone defect: a) PBAT, b) PBAT incorporated with nHA, and c) PBAT incorporated withnHA/CNTs. On the other hand, right tibia critical defects will be filled with clot (control).Each group will be subdivided according to euthanasia period of 2 and 6 weeks (n=10 byperiod). On each five animals from each period, the bone repair area will be assessed through histologic analysis and histomorphometic, meanwhile the other 5 animals the bonerepair area will be submitted to flexion resistance test. The data shall be statistically analyzed, initially through the normality test in order to apply an adequate statistic test. The level of significance adopted will be the conventional value of 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REIS VASCONCELLOS, LUANA MAROTTA; SANTANA-MELO, GABRIELA E.; SILVA, EDMUNDO; PEREIRA, VANESSA FERNANDES; RIBEIRO ARAUJO, JULIANI CAROLINE; ROSA SILVA, ANDRE DINIZ; FURTADO, ANDRE S. A.; VAZ ELIAS, CONCEICAO DE MARIA; VIANA, BARTOLOMEU CRUZ; MARCIANO, FERNANDA ROBERTA; et al. Electrospun Poly(butylene-adipate-co-terephthalate)/Nano- hyDroxyapatite/Graphene Nanoribbon Scaffolds Improved the In Vivo Osteogenesis of the Neoformed Bone. JOURNAL OF FUNCTIONAL BIOMATERIALS, v. 12, n. 1, MAR 2021. (16/04618-7)

SILVA, ALINE DOS SANTOS; MANZOLLI RODRIGUES, BRUNO VINICIUS; OLIVEIRA, FRANCILIO CARVALHO; CARVALHO, JANCINEIDE OLIVEIRA; REIS DE VASCONCELLOS, LUANA MAROTTA; RIBEIRO DE ARAUJO, JULIANI CAROLINE; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. Characterization and in vitro and in vivo assessment of poly(butylene adipate-co-terephthalate)/nano-hydroxyapatite composites as scaffolds for bone tissue engineering. JOURNAL OF POLYMER RESEARCH, v. 26, n. 2, FEB 1 2019. (15/01259-3, 16/04618-7, 11/20345-7, 11/17877-7)