Development and evaluation in vitro of nanofibers scaffolds associated to glycolic extract of Punica granatum (pomegranate)

• Grant number: 16/18646-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2016
• End date: September 30, 2017
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Bruna Emygdio de Melo
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The development of new techniques for the success in endodontic treatment has been searching new technologies. For that, the field of nanotechnology had performed a physical support as scaffolds to hold cells, contributing to the regeneration of dental pulp. Furthermore, the research and development of new phytotherapy materials had been extensively studied due to the increase of microorganisms resistant to conventional antibiotics. This study aims to evaluate the biocompatibility a scaffold associated with the natural extract of Punica granatum (pomegranate). For biocompatibility tests, it will be performed tests on DPSC cells, stem cells from the pulp of permanent teeth. Cytotoxicity will be evaluated by Alamar Blue assay, a colorimetric test that verifying the metabolic activity of the cell in a spectrophotometer (570 nm). For the genotoxicity evaluation will be used the micronucleus test, which analyzes under a nuclear microscope (100X) the changes resulting from chromosomal fragmentation. Statistical analysis will be performed by ANOVA and Tukey's tests (significance level of 5%).