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Use of high-resolution imaging methods for in vivo study of perfusion and myocardial inflammatory changes in an experimental model of chronic chagas cardiomyopathy in hamster

Grant number: 16/16746-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): February 01, 2017
Effective date (End): December 11, 2017
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Marcus Vinicius Simões
Grantee:Luciano Fonseca Lemos de Oliveira
Supervisor abroad: Frank M. Bengel
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : Hannover Medical School, Germany  
Associated to the scholarship:15/25209-5 - Use of high-resolution imaging methods for in vivo study of perfusion and myocardial inflammatory changes in an experimental model of chronic chagas cardiomyopathy in hamster, BP.DR

Abstract

Several pathophysiological aspects of chronic Chagas cardiomyopathy (CCC) still need elucidation, especially the mechanisms that determine the development of chronic myocardial injury only in late stage 2-3 decades after the acute phase. Besides the persistence of the parasite in the cardiac tissue and exacerbate inflammatory response by autoimmunity, experimental and clinical evidences support participation of microvascular ischemia contributing in the process of progression of cardiomyopathy. However, a longitudinal clinical study which allows the observation of these aspects, displays virtual deterrent in front of the long-term observation. In this setting, the use of experimental hamster model that reproduces the chronic phase of CCC after 6 months of infection would provide valuable insights into the pathophysiological mechanisms. Therefore, this study aims to investigate the correlation between myocardial perfusion changes and myocardial inflammation with the progression of myocardial dysfunction in the experimental model of CCC. Briefly, after infection of female hamsters with Y strain of Trypanosoma Cruzi, will be conducted assessments of myocardial performace by echocardiogram-2D, evaluations of myocardial perfusion by use of mini-SPECT and the viability and myocardial inflammation with MicroPET. The assesments of the myocardial function and perfusion will be performed 2, 4, 6, 8 and 10 months after infection. The viability and myocardial inflammation with 18F-FDG will be performed in time of window at 10 months after infection. At the end of the study, all animals suffer euthanasia and histological studies will be conducted to assessment of the inflammation and fibrosis to allow the correlation with the results of studies obtained in vivo. (AU)