Replication stress response characterization in the protozoan Leishmania: the role of the ATR kinase

• Grant number: 16/18192-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2016
• End date: October 31, 2019
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Agreement: BBSRC, UKRI
• Principal Investigator: Luiz Ricardo Orsini Tosi
• Grantee: Matheus Silva e Bastos
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?, AP.TEM

Abstract

In this project we propose to characterize the Leishmania ATR kinase, a central factor in response to replicative stress in other eukaryotes. For this, we will generate an ATR-deficient cell line and carry out the functional characterization of the protein. We will also use high-throughput approaches to study the effect of ATR deficiency in the overall response to replicative stress. For this, we will investigate the effect of replicative stress in the profile of replication origin use and copy number variation across the genome of an ATR-deficient cell line. (AU)