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The effect of biosilicate with two crystalline phases (BioS-2P) on osteoclast differentiation and activity in vivo

Grant number: 16/22528-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2017
Effective date (End): January 31, 2018
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Emanuela Prado Ferraz
Grantee:Ingrid Wenzel Tosin
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Tissue engineering-based therapies for bone defects due to trauma, infections or pathological conditions are of great interesting on oral and maxillofacial and orthopedics field. Among the biomaterials for scaffold purposes, glass ceramics emerges as a good alternative, mainly due their mechanical properties and biocompatibility. Previous results showed that Biosilicate® with two crystalline phases (BioS-2P) is an osteoinductive material capable of increasing osteoblast differentiation, and lead to bone formation when implanted in rat calvaria bone defects, suggesting that this biomaterial may be suitable for use in bone tissue engineering. However, bone remodeling process involves synergistic and controlled actions of formation and resorption, modulated by local and systemic mechanisms, involving other cell types besides the osteoblasts. There are few studies that address the resorption process in the context of tissue engineering and there is no data regarding the effect of BioS-2P on the differentiation and activity of osteoclasts. The aim of our study is to evaluate the effect of scaffolds of BioS-2P on differentiation and osteoclast activity. For this, bone defects created on rat calvaria will be filled with BioS-2P scaffolds for 2 and 4 weeks; the control will be the newly bone on empty defects. The osteoclasts differentiation and activity will be detected by the expression of osteoclast marker genes: receptor activator of nuclear factor kappa B (RANK), metalloproteinase 9 (MMP9), metalloproteinase 13 (MMP13), cathepsin K (CTSK) collagen 10a1 (COL10a1) and tartrate-resistant acid phosphatase (TRAP); also the receptor activator of nuclear factor kappa-B ligand and osteoprotegerina, expressed by osteoblasts. Osteoclast activity will be evaluated on newly bone by TRAP staining. (AU)