Analysis of adhesion, proliferation and protein synthesis by osteoblasts derived from the sutures of smoking rats who suffered jaw orthopedic disjunction

• Grant number: 16/17919-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2017
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Maria Bernadete Sasso Stuani
• Grantee: Gabriel Dessotti Barretto
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Introduction: Smoking is a common habit and, most often, begins in adolescence. Among the various systemic changes caused, the commitment of the cellular repair process is of great importance in the comprehension of dento-skeletal movements. There is no scientific evidence to relate this habit and their influence on orthodontic/orthopedic treatment, especially in regard to cellular and molecular analyzes. The osteoblast is the most important cell in the process and the success of bone formation depends on their cell adhesion and proliferation. Objectives: Our goals will be to analyze the adhesion, proliferation and protein synthesis by osteoblasts derived from the sutures of smoking rats who suffered jaw orthopedic disjunction. Matherial and Methods: A total of 60 male Wistar rats are divided into 4 groups (n=15): negative control [intact suture, smokeless cigarette (FC)], experimental group I (intact and with FC suture), II experimental group [rapid maxillary expansion (RME) and non-CF] and experimental group III (RME + FC). Each group is divided into 3 experimental evaluation periods (n=5) and the animals of each subgroup sacrificed after 3, 7 and 10 days after orthopedic jaw expansion. Exposure to smoke will be made for 21 days before the RME. For the analysis of cell proliferation, growth curves will be conducted with explanted osteoblasts, after the jaw disjunction and grown in culture media. The analysis of osteoblast adhesion rate is performed according to the technique described by Karu et al, 1996, with some modifications. All experiments will be conducted in triplicate and the results will be grouped according to different groups of study and experimental period, submitted to analysis of variance and compared by the Student t test. The difference will be considered statistically significant when p <0.05. (AU)