Salivary histatins degradation in Down Syndrome subjects and antibacterial influence on periodontopathogens

• Grant number: 17/00931-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 01, 2017
• End date: April 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Elisa Maria Aparecida Giro
• Grantee: Natália Bertolo Domingues
• Supervisor: Walter Luiz Siqueira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: Western University , Canada
• Associated to the scholarship: 15/25294-2 - Salivary histatins degradation in Down syndrome subjects and antibacterial influence on periodontopathogens, BP.DR

Abstract

The aims of this study are to evaluate (1) proteolysis of histatins 1 and 5 in whole saliva of subjects with Down syndrome (DS) and periodontal disease (PD), and (2) the antimicrobial activity of degradation products against periodontopathogens. Twenty four subjects will be selected and allocated in the following groups (n=6): DS with PD, DS without PD, non-syndromic with PD and non-syndromic without PD (control group). After stimulated whole saliva collection, samples will be centrifuged in order to obtain the supernatant of whole saliva (WSS), aliquoted and stored at -80°C. Protein degradation will be performed by adding synthetic histatins 1 or 5 to pure WSS and diluted WSS (1:10). The samples will be incubated at 37°C for 0, 0.5, 1.5, 4, 6, 8, 24 and 48 hours. Next, reversed-phase high performance liquid chromatography (RP-HPLC), polyacrylamide cationic gel electrophoresis (PAGE) and mass spectrometry (MS) will be performed. Statistical tests will be defined based on variation factors of the experiments and on data distribution and homoscedasticity. The significance level will be set at 5%. (AU)