The effects of ionizing radiation on the distribution of cysteine-cathepsins in restored and sound dentin

• Grant number: 17/01777-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: June 01, 2017
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Ana Cecília Corrêa Aranha
• Grantee: Sandra Ribeiro de Barros da Cunha
• Supervisor: Annalisa Mazzoni
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Università di Bologna, Italy
• Associated to the scholarship: 15/20297-3 - The effects of radiotherapy on the endogenous proteases of sound and restored dentin, BP.DR

Abstract

To investigate in vitro the effect of ionizing radiation on the CTs of restored and sound dentin, 40 human third molars will be randomly divided into 4 different groups (n=10): Sound and Non-Irradiated (G1); Sound and Irradiated (G2); Restored and Non-Irradiated (G3) and Restored and Irradiated (G4). Specimens of irradiated groups will receive a 70Gy dose of radiation. Specimens of restored groups will be restored with Adper Single Bond 2 and Clear Fill SE Bond. Samples will be prepared in accordance with Mazzoni et al., 2009 e Scaffa et al., 2017 for the Immunohistochemical with correlative FEI-SEM and TEM. Images will be taken in areas considered as representative for each group and acquired using FEI-SEM or TEM (JSM 890, JEOL, Tokyo, Japan). Two independent observers in a blind study design will analyze the images. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CUNHA, SANDRA RIBEIRO; MARAVIC, TATJANA; COMBA, ALLEGRA; RAMOS, PEDRO AUGUSTO; TAY, FRANKLIN R.; PASHLEY, DAVID H.; FREGNANI, EDUARDO RODRIGUES; ARANHA, ANA CECILIA; MAZZONI, ANNALISA; BRESCHI, LORENZO. In vivo and in vitro radiotherapy increased dentin enzymatic activity. Journal of Dentistry, v. 100, p. 6-pg., 2020-09-01. (15/20297-3, 17/01777-0)