Pulp tissue stem cells: culture, characterization and differentiation

• Grant number: 17/07687-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2017
• End date: May 31, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Fábio Dupart Nascimento
• Grantee: Agatha Maria Pelosine
• Host Institution: Pró-Reitoria Acadêmica. Universidade de Mogi das Cruzes (UMC). Campus da Sede Mogi das Cruzes. Mogi das Cruzes , SP, Brazil
• Associated research grant: 13/05822-9 - Proteinase-Activated Receptors (PARs) in the dentin-pulp complex: identification, modulation and signal tranduction in caries disease, AP.JP

Abstract

The heyday of regenerative medicine has a title that is well known, stem cells. In dental medicine, several studies converge to the development of mechanisms that use stem cells for and replacement of buccal tissues, since dental and periodontal tissue loss are common abnormalities that irreversibly threaten the general health of the patient. However, so far, the protocols for rehabilitation of the stomatognathic system is basically non-biological. The present project aims to establish techniques of acquisition, differentiation and characterization of odontoblasts from stem cells originated from the dental pulp. So, we will use techniques of cell culture, extraction and purification of genetic material, gene and protein expression analysis using confocal scanning laser microscopy. With this project, it is expected that a new line of research, which unites the dental clinic and the basic research lab recently installed in our Institution. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA ROCHA, ELAINE A.; ALVAREZ, MARCELA M. P.; PELOSINE, AGATHA M.; CARRILHO, MARCELA ROCHA O.; TERSARIOL, IVARNE L. S.; NASCIMENTO, FABIO D.. Laser Photobiomodulation 808nm: Effects on Gene Expression in Inflammatory and Osteogenic Biomarkers in Human Dental Pulp Stem Cells. FRONTIERS IN PHARMACOLOGY, v. 13, p. 9-pg., 2022-01-17. (13/05822-9, 17/07687-2)