Follicular system: a new protocol for oocyte in vitro maturation. effects on complex cumulus-oocyte differentiation and embryo production

• Grant number: 16/21671-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2017
• End date: February 29, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: José Buratini Junior
• Grantee: Ana Caroline Silva Soares
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The oocyte in vitro maturation (IVM) is one of the main bottlenecks in vitro production of bovine embryos (IVP). The existing protocols for IVM do not reflect the physiological conditions and determine mismatch between nuclear and cytoplasmic maturation of the oocyte, thus compromising their fertility. Oocytes undergoing IVM are from follicles in different stages of development and this heterogeneity hinders the establishment of ideal growing conditions. The aim of this project to develop a new system for IVM, follicular system, which includes a strategy for synchronization of follicular activity in donor cow in order to obtain homogeneous population of oocytes, as well as sequential cropping system adjusted to the maturation phase of oocytes recovered. The experiments included in the project aimed at developing / processing protocols for ovarian synchronization donor cows and for crops sequences which mimic the conditions intrafollicular before (pre-IVM phase) and during ovulation (IVM phase). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA SOARES, ANA CAROLINE; SAKODA, JHESSICA NAOMI; GAMA, ISABELA LIMA; BAYEUX, BERNARDO MARCOZZI; LODDE, VALENTINA; LUCIANO, ALBERTO MARIA; BURATINI, JOSE. Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds. Theriogenology, v. 141, p. 146-152, JAN 1 2020. (17/07588-4, 16/21671-9)

SILVA SOARES, ANA CAROLINE; GOMES MARQUES, KELLY NADER; MARTIGNONI BRAGANCA, LUIZ GUSTAVO; LODDE, VALENTINA; LUCIANO, ALBERTO MARIA; BURATINI, JOSE. Synchronization of germinal vesicle maturity improves efficacy of in vitro embryo production in Holstein cows. Theriogenology, v. 154, p. 53-58, SEP 15 2020. (17/07588-4, 16/21671-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOARES, Ana Caroline Silva. Follicular system: a new protocol for oocyte in vitro maturation. Effects on cumulus-oocyte complex differentiation and embryo production. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.