Effects of lithium chloride on the ligature-induced bone loss in rats with and without estrogen deficiency

• Grant number: 16/23614-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2017
• End date: March 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Magda Feres Figueiredo
• Grantee: Fernando de Souza Malta
• Host Institution: Universidade Universus Veritas Guarulhos (Univeritas UNG). Guarulhos , SP, Brazil

Abstract

The aim of the present study will be to evaluate the effects of lithium chloride, a drug able of affecting bone cells in favor of bone formation being a potential candidate for treatment of osteoporosis, in the ligature-induced bone loss in rats with and without estrogen deficiency. Ninety-six Wistar rats (24 rats per group / 8 per experimental time) will be allocated into one of the following groups: 1 - sham surgery and administration of saline solution by gavage; 2- ovariectomy and administration of saline solution by gavage; 3- sham surgery and administration of 200 mg/kg/day of lithium chloride by gavage; 4- ovariectomy and administration of 200 mg/kg/day of lithium chloride by gavage. Administration of lithium chloride or saline solution will be introduced at 14 days after ovariectomy/sham procedures and will be prolonged until the end of the experiment. Twenty-one days after ovariectomy/sham, the animals will receive a ligature in one of the mandibular first molars while the contralateral first molar will be left without ligature to work as a control for the ligature-induced bone loss. The animals will be euthanized at 10, 20 and 30 days after the ligature placement. The mandibles will be removed and processed to obtain decalcified specimens containing the first molars, which will be used for histometric, histochemical and immunohistochemical evaluations. Stereometric analysis will include counts of the cells related to the inflammatory process in the lesion. Histometric assessments will include measurements of bone loss and bone density in the bifurcation region. Histochemical and immunohistochemical evaluations will include the counts of cells positive for tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), osteopontin (OPN) and osteocalcin (OCN) in the bone tissue of the region of the furcation. In the statistical analysis, the results will first be submitted to the normality test (Shapiro-Wilk). Subsequently, histometric, histochemical and immunohistochemical data will be compared by parametric (Analysis of Variance) or non-parametric (Kruskal-Wallis) tests and, in case of differences, by their respective post hoc tests. Comparisons between ligated and non-ligated teeth will be performed by the paired t-test or Wilcoxon. A significance level of 5% will be set for all analyses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MALTA, FERNANDO DE SOUZA; NAPIMOGA, MARCELO HENRIQUE; MARINS, LETICIA MACEDO; MIRANDA, TAMIRES SZEREMESKE; DE OLIVEIRA, FLAVIANNY BARBARA; POSCH, ALINE TANY; FERES, MAGDA; DUARTE, POLIANA MENDES. Lithium chloride assuages bone loss in experimental periodontitis in estrogen-deficient rats. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 6, p. 2025-2036, JUN 2020. (16/23614-2)