Massive sequencing of an amplicons library to obtain nuclear and plastid data in order to infer phylogenetic relationships of genus Cereus (Cactaceae) species

• Grant number: 17/07017-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2017
• End date: June 30, 2018
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Fernando de Faria Franco
• Grantee: Fernanda Mayumi Pinho Kawauti
• Host Institution: Centro de Ciências Humanas e Biológicas (CCHB). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil

Abstract

The species from family Cactaceae are associated to arid and semi-arid climates, becoming attractive models to study evolutionary process in xeric environments. The main goal of this project is isolate molecular data from nuclear and plastid genomes, which might be useful to infer phylogenetic relationships of genus Cereus (Cactaceae) species. Thus, it will be performed the cross-amplification of primers set previously described to amplify plastid segments and know genes as well as some anonymous nuclear loci recently described for the cactus genus Pilosocereus. To get molecular information an amplicons library will be prepared according protocols available in literature, followed by massive DNA sequencing in MiSeq Illumina platform. The generate data will be analyzed following traditional phylogenetic (Maximum Likelihood and Bayesian Inference) and coalescent-based analyses (Species Tree). Overall, this project should contribute for both the standardization of the method to prepare amplicons library as well as to elucidate phylogenetic relationships of genus Cereus. (AU)