Gene expression of immunity markers of human keratinocytes cultured of severe burn injury

• Grant number: 16/17176-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2017
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alfredo Gragnani Filho
• Grantee: Wagner Luis Marson
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Burns affect about one percent of the world population. More than one million burns occur in the United States each year and about 5,000 are fatal. This classifies the burn as the fourth leading cause of death from unintentional injuries in this country. Patients victims of burns have high susceptibility to infections, being directly related to morbidity and mortality of the group. PURPOSE: Evaluate the expression profile of genes related to Innate and Adaptive Immune System of human primary epidermal keratinocytes and fragments of skin of patients with severe burns. METHODS: After obtaining viable fragments of skin with and without burning, culture of human primary epidermal keratinocytes will be initiated by the enzymatic method using Dispase. These cells will be treated with Trizol® for extraction of total RNA. This will be quantified and analyzed for purity for obtaining cDNA for the analysis of gene expression using specific PCR Arrays plates. (AU)