Use of DNA metabarcoding as alternative, resolute and low-cost tool to DNA barcoding in ecological studies

• Grant number: 17/12758-6
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: September 01, 2017
• End date: November 30, 2017
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Claudio de Oliveira
• Grantee: André Batista Nobile
• Supervisor: Juan Pedro M. Camacho
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Universidad de Granada (UGR), Spain
• Associated to the scholarship: 15/19025-9 - Identification of priority areas for the conservation of threatened species in the Mogi-Guaçu river using techniques of DNA barcode, BP.PD

Abstract

Studies involving ichthyoplankton are of great value for management projects of endangered species. However, correct identification of eggs and larvae is a difficult task even for specialists. In this sense, the use of molecular tools, such as DNA barcoding, has been shown to be an effective and resolutive alternative in such studies, increasing its effectiveness. However, in large-scale studies with many samples, the cost of DAN Barcode analysis and the time required to perform the entire molecular process, makes its use impracticable. So, the use of Metabarcoding may be a more affordable and fast alternative, maintaining the assertiveness of the studies. Thus, the objective of this project is to develop a protocol for analysis of eggs and larvae using the DNA Metabarcoding, so that it can be used on a large scale in future studies with eggs and larvae.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA COSTA, GABRIELA O.; NOBILE, ANDRE B.; LIMA, FELIPE P.; FREITAS-SOUZA, DIOGO; MANZANO, FELIPE V.; OLIVEIRA, CLAUDIO. Length-weight and length-length relationships for four fish species of Upper Paranaiba river basin, Brazil. Journal of Applied Ichthyology, v. 35, n. 2, p. 619-621, APR 2019. (16/09204-6, 14/26508-3, 15/19025-9, 17/12758-6)

FREITAS-SOUZA, DIOGO; NOBILE, ANDRE BATISTA; DO PRADO, FERNANDA DOTTI; SERRANO, ERICA ALVES; LIMA, FELIPE PONTIERI; FORESTI, FAUSTO; PORTO-FORESTI, FABIO; OLIVEIRA, CLAUDIO. Genetic markers indicate that hybrids of Pseudoplatystoma (Siluriformes, Pimelodidae) are reproducing in natural environments in southeastern Brazil. Biological Invasions, v. 24, n. 4, p. 10-pg., 2022-01-16. (17/12758-6, 15/19025-9, 16/09204-6, 14/26508-3, 18/20610-1)