Correlation between alkaline phosphatase of seminal plasma and sperm parameters in buffalos

• Grant number: 17/11224-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2017
• End date: July 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Eunice Oba
• Grantee: Tatiana Cezar Fernandes da Silva
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The seminal plasma components play a role to modulate the functions and quality of sperm cells. Enzymes as the alkaline phosphatase (AP) have been reported to participate in the spermatozoa metabolism during their epididymal transit. As alkaline phosphatase is produced in high concentrations in the epididymis can be used as a marker to identify the complete ejaculation, oligospermia and azoospermia in many species. Thus, the aim of this study was to assess the correlation between the AP and sperm parameters in buffalos. Fifteen healthy buffalos were used, with 3 to 5 years-old created in extensive. The semen samples were collected by electroejaculation and seminal plasma was separated by centrifugation at 900xg/10 min to perform AP analysis. The subjective total motility (0-100%) and sperm concentration in Neubauer chamber were determined. The seminal plasma AP concentration was evaluated by automatic method and (Cobas Mira Plus, Roche Diagnostic Systems and Bioclin Kit, Kinetic, K021, REF Laboratory Quibasa Chemical Basici Ltda. Statistical analysis was performed using Sigma Plot 11.0 software, using Shapiro-Wilk test (normality test) and Spearman correlation. The data was represented by median and quartiles 25% and 75%.