Study the correlation between P2X7 receptor and inflammation markers in diabetic rats kidneys

• Grant number: 16/24917-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2017
• End date: June 30, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Elisa Mieko Suemitsu Higa
• Grantee: Camila Farias
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Data of 2008 disclosed by the World Health Organization affirm that diabetes mellitus was diagnosed in approximately 347 million people, which can be doubled by 2030. Diabetes is a chronic disease that occurs when there is insufficient insulin production or when it can not be used efficiently by the body. The P2X7 receptor (P2X7R) is synthesized in situations where high amounts of ATP are released, particularly under pathological conditions, such as in diabetes. A previous study conducted in our laboratory showed that production and activation of P2X7R were associated with oxidative stress in diabetes; however, it was not analyzed whether this receptor could be associated with inflammatory factors in the progression of this disease. Objective: correlate P2X7 and inflammatory markers in the kidneys in diabetic rats. Methods: Male Wistar rats will be nephrectomized unilaterally. Half of the animals will be submitted to the single application of streptozotocin (60 mg / kg, i.v.) for induction of diabetes, constituting the diabetic group (DM) and the other half will receive the drug vehicle, forming the control group (CTL). The animals will be euthanized at the 5th, 6th, 7th and 8th weeks of diabetes and the kidneys will be removed and cut longitudinally; part of the tissue will be used for protein analysis via Western blotting, with antibodies against IL-6, IL-10, TNF-±, NF-kB p65, TGF-² and P2X7, all will be standardized by actin. The other part of renal tissue will be used for histological analysis (HE and PAS). The results will be described as mean ± standard error, with statistical significance of p <0.05. (AU)