EPPIN interacting proteins on mouse sperm surface: identification, expression and potential physiological roles

• Grant number: 17/11363-8
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): August 01, 2017
• Effective date (End): July 31, 2019
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Erick José Ramo da Silva
• Grantee: Noemia Aparecida Partelli Mariani
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 15/08227-0 - A study on EPPIN as a drug target for male contraception: developing a new animal model to test the efficacy of male contraceptive drugs, AP.JP

Abstract

The EPPIN gene (Epididymal protease inhibitor) is a member of the superfamily of protease inhibitors, recognized as a promising target for male contraception due to its critical role on fertility. EPPIN is expressed and secreted by Sertoli cells and epididymal epithelial cells and covers the surface of human spermatozoa, where it works as a modulator of sperm motility after ejaculation by the interacting with the seminal plasma protein Semenogelin-1 (SEMG1). Little is known, however, about the mechanisms by which the EPPIN modulates the sperm motility in vivo, as well as its role in other functions of the male reproductive tract. Uncover how this gene regulates male fertility is, at this moment, one of the objectives of our laboratory. In this project, we propose to identify the interaction proteins of EPPIN In the male reproductive tract of the mouse (Mus musculus). We will perform proteomic assays to indentify EPPIN interacting proteins in the epididymis and on sperm surface from adult mice, as well as Western blot and Immunohistochemistry assays, to evaluate the expression and cellular distribution of EPPIN binding partners. The results of this project will contribute to broaden our knowledge about evolutionary aspects of the EPPIN, its physiological roles in male reproductive tract, as well as to the development of pharmacological strategies to control of the male fertility. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARIANI, NOEMIA A. P.; CAMARA, ALINE C.; SILVA, ALAN ANDREW S.; RAIMUNDO, TAMIRIS R. F.; ANDRADE, JULIANA J.; ANDRADE, ALEXANDRE D.; ROSSINI, BRUNO C.; MARINO, CELSO L.; KUSHIMA, HELIO; SANTOS, LUCILENE D.; et al. Epididymal protease inhibitor (EPPIN) is a protein hub for seminal vesicle-secreted protein SVS2 binding in mouse spermatozoa. Molecular and Cellular Endocrinology, v. 506, APR 15 2020. (17/11363-8, 16/23025-7, 15/08227-0)

SILVA, ALAN A. S.; RAIMUNDO, TAMIRIS R. F.; MARIANI, NOEMIA A. P.; KUSHIMA, HELIO; AVELLAR, MARIA CHRISTINA W.; BUFFONE, MARIANO G.; PAULA-LOPES, FABIOLA F.; MOURA, MARCELO T.; SILVA, ERICK J. R.. issecting EPPIN protease inhibitor domains in sperm motility and fertilizing ability: repercussions for male contraceptive developmen. MOLECULAR HUMAN REPRODUCTION, v. 27, n. 12, DEC 2021. (19/13661-1, 15/08227-0, 17/11363-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARIANI, Noemia Aparecida Partelli. EPPIN interaction proteins in mouse sperm: identification, expression and potential physiological roles. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.