EFFECTS OF BONT-A PERIPHERAL-APPLICATION ON CENTRAL SENSITIZATION IN TMJ OF RATS

• Grant number: 17/07741-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2017
• End date: February 29, 2020
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Altair Antoninha Del Bel Cury
• Grantee: Victor Ricardo Manuel Muñoz Lora
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated scholarship(s): 18/13575-5 - Involvement of the glial cells in the antinociceptive effect of Botulinum toxin type A, BE.EP.DR

Abstract

It has been demonstrated that Botulinum toxin type A (BoNT-A), in addition of inhibiting the release of acetylcholine at the neuromuscular junction, has a peripheral antinociceptive effect that also reduces the release of sensitive neurotransmitters such as substance P, glutamate, calcitonine gene related peptide and the pro-inflammatory cytokine IL-1².(1) Moreover, current data indicate a possible central effect of BoNT-A, influencing the processes of interaction between neuronal and glial cells, considered as the key underlying chronic pain maintenance.(2,3) This study aims to investigate, in an animal model, whether intra-articular application of BoNT-A is able to act centrally on the nociception induced by a chronic inflammatory process, particularly by modulating glial and neuronal cells interactions. To achieve this objective, Wistar rats will be induced to persistent hypernociception in the left TMJ, and then treated with intra-articular injections of BoNT-A (7U / kg) in the compromised TMJ.(1) Finally, samples of peri-articular tissue, trigeminal ganglion and subnucleus caudalis will be collected 24 hours, 7 and 14 days after treatment application, and submitted to molecular tests of Elisa (Fractalkine, Cathepsin S), Western Blot (CD11b, CX3 Chemokine Receptor 1, P2X Receptor 7, and P38 Mitogen Activated Protein Kinase) and Immunohistochemistry (cleaved-SNAP25, P2X Receptor 7, and Transient Receptor Potencial Vanilloid 1). Data will be collected and the corresponding statistical analysis will be applied.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANUEL MUNOZ-LORA, VICTOR RICARDO; OKROSA, ANA DUGONJIC; MATAK, IVICA; DEL BEL CURY, ALTAIR ANTONINHA; KALINICHEV, MIKHAIL; LACKOVIC, ZDRAVKO. Antinociceptive Actions of Botulinum Toxin A1 on Immunogenic Hypersensitivity in Temporomandibular Joint of Rats. TOXINS, v. 14, n. 3, p. 17-pg., 2022-03-01. (18/13575-5, 17/07741-7)

MANUEL MUNOZ-LORA, VICTOR RICARDO; ABDALLA, HENRIQUE BALLASSINI; DEL BEL CURY, ALTAIR ANTONINHA; CLEMENTE-NAPIMOGA, JULIANA TRINDADE. Modulatory effect of botulinum toxin type A on the microglial P2X7/CatS/FKN activated-pathway in antigen-induced arthritis of the temporomandibular joint of rats. Toxicon, v. 187, p. 116-121, NOV 2020. (17/07741-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LORA, Victor Ricardo Manuel Muñoz. Clinical applications and central effects of Botulinum toxin type A in the trigeminal region. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.