Synthesis and evaluation of biomaterials associated with extracellular matrix proteins for the pulp tissue regeneration

• Grant number: 17/14210-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2017
• End date: June 30, 2021
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Maria Luísa de Alencar e Silva Leite
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 19/14965-4 - Synthesis, characterization and biological evaluation of a mineral-coated porous chitosan scaffold for pulp-dentin complex regeneration, BE.EP.DR

Abstract

The aim of this study is to develop experimental nanofibrous scaffolds and hydrogel containing extracellular matrix proteins capable of participating effectively of the regenerative process of pulp tissue through biostimulation of human Stem Cells from Apical Papilla (SCAPs). In study 1, the optimum concentration of extracellular matrix proteins (fibronectin, laminin and collagen I) that present bioactivity on SCAPs cells will be determined according to the following parameters: cell viability and proliferation; cell adhesion and spread; cell migration; specific genes expression; and collagen synthesis. In study 2, different formulations of scaffolds based on poly([-caprolactone) with parallel and random arrangement of the nanofibers will be assessed, evaluating the surface topography of the scaffolds, their resistance to degradation as well as the potential to induce cell adhesion, spreading, and proliferation. After selecting the best scaffold formulation with parallel and random arrangement of nanofibers, its biological effect associated with extracellular matrix proteins, based on the results of the study 1, will be evaluated. In study 3, different formulations of a hydrogel based on gelatin will be tested, evaluating their handling characteristics, as well as, their potential to induce cell adhesion, spreading and proliferation. After selecting the best hidrogel formulation, its biological activity associated with extracellular matrix proteins, based on the results of study 1, will be evaluated. Finally, in study 4 the formulated biomaterials (scaffold of nanofibres and hydrogel) will be assessed using an innovative in vitro model of pulp regeneration, in which roots obtained from human teeth will individually adapted to perfusion artificial pulp chambers, under dynamic intrapulpal pressure. Histological and immunohistochemical analyzes will be carried out in order to determine the tissue morphology and markers expression of collagen synthesis and odontogenic differentiation along the root canal. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; ANOVAZZI, GIOVANA; ANSELMI, CAROLINE; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Signaling Biomaterial for Dental Pulp Regeneration. JOURNAL OF ENDODONTICS, v. 47, n. 7, p. 1110-1117, JUL 2021. (17/14210-8, 17/22739-9)

LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; ANOVAZZI, GIOVANA; MENDES SOARES, IGOR PAULINO; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Development of fibronectin-loaded nanofiber scaffolds for guided pulp tissue regeneration. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 109, n. 9, p. 1244-1258, SEP 2021. (17/22739-9, 17/14210-8)

LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; ANOVAZZI, GIOVANA; WU MON, FILIPE KOON; BORDINI, ESTER ALVES FERREIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Bioactivity effects of extracellular matrix proteins on apical papilla cells. Journal of Applied Oral Science, v. 29, 2021. (17/22739-9, 17/14210-8)

LEITE, MARIA LUISA; DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; SOARES, DIANA GABRIELA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Poly(caprolactone)-aligned nanofibers associated with fibronectin-loaded collagen hydrogel as a potent bioactive scaffold for cell-free regenerative endodontics. International Endodontic Journal, v. 55, n. 12, p. 13-pg., 2022-09-10. (17/14210-8, 17/22739-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LEITE, Maria Luísa de Alencar e Silva. Synthesis and evaluation of biomaterials associated with extracellular matrix proteins for pulp tissue regeneration. 2021. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.