Histomorphological evaluation of periodontal treatment associated with photodynamic therapy in the modulation of periodontal and lung inflammation in an experimental model of acute asthma

• Grant number: 17/14139-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2017
• End date: September 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Anna Carolina Ratto Tempestini Horliana
• Grantee: Jessica Gonzaga de Oliveira
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

Asthma and periodontal disease (PD) present high prevalence and a high economic and social cost. Evidence suggests that PD can exert systemic immunomodulatory effects. As the standard periodontal treatment (PD) for PD may not completely eliminate periodontopathogens from photodynamic therapy (PDT), they have been used with promising results. Therefore, the objective of this study will be to evaluate if PD associated with PDT is able to modulate pulmonary inflammation in an experimental model of acute asthma. Forty male Balb / c mice (~ 2 months) were divided into 8 groups (n = 5): (1) Basal - unmanaged; (2) with DP; (3) with DP + TP; (4) with DP + TP + PDT, (5) Asthma, (6) A + DP, (7) A + DP + TP, (8) A + DP + TP + PDT. PD was induced by ligation technique (15 days) and asthma by administration of ovalbumin (OVA) and aluminum hydroxide, subcutaneously (days 0 and 7) and nebulization with OVA (3 x / week, for 2 weeks). TP was performed with mini-five curettes and PDT with methylene blue (0.005%), l = 660 nm, energy density 6,369 J / cm², 9J per point at 2 points. After 21 days the animals were euthanized for morphological analysis of the lung and the mandible. (AU)