Evaluation of Parameters of Tissue Dosimetry in Photodynamic Therapy for the Treatment of Periodontal Disease - clinical study

• Grant number: 16/10269-5
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2016 - February 28, 2019
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Renato Araujo Prates
• Grantee: Renato Araujo Prates
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated researchers: Daniela de Fátima Teixeira da Silva ; Ilka Tiemy Kato Prates ; Martha Simões Ribeiro ; Sandra Kalil Bussadori ; Verônica Franco de Carvalho

Abstract

Periodontal disease is an inflammatory disease of the support tissue of the teeth induced by microorganisms and influenced by immune-inflammatory response of the host. Mechanical periodontal treatment based on scaling and root planning can often not completely remove the periodontal pathogens and thus require the adjuvant use of antibiotics. In order to reduce the use of antibiotics, antimicrobial photodynamic therapy (PDT) may be used as adjunct to conventional periodontal treatment. However, studies show many discrepancies between the parameters adopted for the PDT, making it difficult to compare results and measure its efficacy. The objective of this research is to investigate the dosimetric parameters used in PDT for the treatment of periodontal disease. This clinical trial will include 20 patients with chronic periodontitis according to the eligibility criteria. Patients in periodontal treatment will be recruited from Dental Clinic of Nove de Julho University, where the research will be developed. Three incisors will be selected form each patient for experimental procedures. Initially, the pattern of light scattering will be recorded by image with intraoral photographs. Then, photodynamic therapy will be performed with three irradiation periods of 1, 3 and 5 min. It will be used laser with wavelength of 660 nm and 100 mW of power. Methylene Blue will be used as photosensitizer at a concentration of 100 µM. The concentration of methylene blue in periodontal pockets will be assessed by spectrophotometer before irradiation. Furthermore, microbiological evaluation will be performed from samples of subgingival periodontal pockets of experimental sites before and immediately after the irradiation procedures. The data will be analyzed statistically. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVARENGA, LETICIA H.; GOMES, ANA CAROLINA; CARRIBEIRO, PAMELA; GODOY-MIRANDA, BIANCA; NOSCHESE, GIOVANE; RIBEIRO, MARTHA SIMOES; KATO, ILKA TIEMY; BUSSADORI, SANDRA KALIL; PAVANI, CHRISTIANE; ELOY GERALDO, YHAGO GAZOTE; et al. Parameters for antimicrobial photodynamic therapy on periodontal pocket-Randomized clinical trial. Photodiagnosis and Photodynamic Therapy, v. 27, p. 132-136, SEP 2019. (16/10269-5)

ALVARENGA, LETICIA HEINECK; RIBEIRO, MARTHA SIMOES; KATO, ILKA TIEMY; NUNEZ, SILVIA CRISTINA; PRATES, RENATO ARAUJO. Evaluation of red light scattering in gingival tissue - in vivo study. Photodiagnosis and Photodynamic Therapy, v. 23, p. 32-34, SEP 2018. (16/10269-5)

LEAL, CINTIA R. LIMA; ALVARENGA, LETICIA H.; OLIVEIRA-SILVA, TAMIRES; KATO, ILKA T.; GODOY-MIRANDA, BIANCA; BUSSADORI, SANDRA K.; RIBEIRO, MARTHA S.; PRATES, RENATO A.. Antimicrobial photodynamic therapy on Streptococcus mutans is altered by glucose in the presence of methylene blue and red LED. Photodiagnosis and Photodynamic Therapy, v. 19, p. 1-4, SEP 2017. (16/10269-5)

BELINELLO-SOUZA, ESTEFANI L.; ALVARENGA, LETICIA H.; LIMA-LEAL, CINTIA; ALMEIDA, PATRICIA; LEITE, CAROLINA GUIMARAES; LIMA, TAIRINE R.; GODOY-MIRANDA, BIANCA; PREVIATI-OLIVEIRA, JHOSEPHER; DE PRETTO, LUCAS; DE FREITAS, ANDERSON ZANARDI; et al. Antimicrobial photodynamic therapy combined to periodontal treatment: Experimental model. Photodiagnosis and Photodynamic Therapy, v. 18, p. 275-278, JUN 2017. (16/10269-5)

FRUET-ARRUDA, REGINA T.; ANSELMO, GIULIANA GIOVINAZZO; TORTAMANO, ANA CAROLINA A. C.; ROSSI, ADRIANA L.; BIFFI, MARIA BEATRIZ; MARCO, RODRIGO LABAT; DA SILVA, DANIELA DE F. T.; KATO, ILKA T.; NUNEZ, SILVIA C.; PRATES, RENATO A.. Melanin pigmented gingival tissue impairs red-light lateral scattering for antimicrobial photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 33, MAR 2021. (16/10269-5)

ANSELMO, GIULIANA GIOVINAZZO; TORTAMANO, ANA CAROLINA ALVES CAMARGO; GONCALVES, MARCELA LETICIA LEAL; LEAL-ROSSI, ADRIANA; GODOY-MIRANDA, BIANCA APARECIDA; OLIVEIRA, MARCIA REGINA CABRAL; OLIVEIRA, PEDRO HENRIQUE CABRAL; ALVES, CAROL BRANDT; BUSSADORI, SANDRA KALIL; PRATES, RENATO ARAUJO. Antimicrobial photodynamic chemotherapy mediated by PapaMBlue on chronic periodontal disease Study protocol for a randomized, blind, controlled trial. MEDICINE, v. 99, n. 6, FEB 2020. (16/10269-5)

DE OLIVEIRA-SILVA, TAMIRES; ALVARENGA, LETICIA H.; LIMA-LEAL, CINTIA; GODOY-MIRANDA, BIANCA; CARRIBEIRO, PAMELA; SUZUKI, LUIS CLAUDIO; RIBEIRO, MARTHA SIMOES; KATO, ILKA TIEMY; PAVANI, CHRISTIANE; PRATES, RENATO A.. Effect of photodynamic antimicrobial chemotherapy on Candida albicans in the presence of glucose. Photodiagnosis and Photodynamic Therapy, v. 27, p. 54-58, SEP 2019. (16/10269-5)